Selection of Birch Trees Towards Resilience Against High Salinity Based on Cells Heterogeneity in Callus Cultures of an In Vitro Collection

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

In the context of climate change and increasing soil salinization, research on adaptive breeding is becoming especially relevant. In vitro stress modelling is a promising biotechnological approach to plant selection for resistance to negative environmental factors. The effect of sodium chloride salinization (to simulate salt and osmotic stress) in vitro on the survival, growth, callusogenesis, and morphogenesis of 12 collection clones of Betula pendula L., B. pubescens Ehrh., B. pendula Roth var. carelica (Mercklin) Hämet-Ahti, B. pendula “dalekarlica” (L.f.) was studied. A significant influence of genotype on the survival of explants and their ability to regenerate under salt exposure was demonstrated. It has been established that the use of a 4-fold stepwise (from 0.2 to 1 %) increase in NaCl exposure on a selective nutrient medium without hormones allows differentiating clones by salinity resistance. The highest adaptive capabilities under NaCl-induced salinity were shown by the clones of downy birch, the lowest — by the clones of silver birch. Informative and relatively simple indicators for differentiating clones are the frequency of callus formation and the viability of callus cultures, which characterise the initial and final phases of callus formation and depend on the genotypic characteristics of the original plants. A selective in vitro system is presented, based on the genetic diversity of collection clones (species, varieties, polyploids, mixoploids) and induced cellular heterogeneity of stem callus cultures obtained from the clones most resilient against salinity. The results of the studies confirm the potential of using genetic variability of the initial material under conditions of simulated NaCl stress for selection of birch clones (and lines) adapted to salinity. Three regenerated birch lines selected using selective nutrient media in vitro are promising initial material for adaptive selection.

Авторлар туралы

O. Mashkina

Research Institute of Forest Genetics, Breeding and Biotechnology, Federal Forestry Agency; Voronezh State University

Email: mashkinaos@mail.ru
Lomonosov st. 105, Voronezh, 394087 Russia; Universitetskaya sq. 1, Voronezh, 394018 Russia

T. Tabatskaya

Research Institute of Forest Genetics, Breeding and Biotechnology, Federal Forestry Agency

Хат алмасуға жауапты Автор.
Email: mashkinaos@mail.ru
Lomonosov st. 105, Voronezh, 394087 Russia

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