Study of heme and globin conformation in fractionated rat erythrocytes by means of raman spectroscopy

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Abstract

Conformational changes of heme and globin in fractionated rat erythrocytes have been investigated using Raman spectroscopy. The results obtained show that normal isoforms (common variants) of hemoglobin, which plays a key role in oxygen transport and a protective role against oxidative stress, are dominant (more than 80%) in rat blood. However, heavy chain (3%) and light chain isoforms (11%) formed as a result of protein polymerization or degradation perform important roles in the body because they also function in signal transmission and binding to exogenous ligands.

About the authors

B. G Yushkov

Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences;GAUZ SO “Institute for Medical Cell Technologies”

Yekaterinburg, Russia

M. G Zuev

Institute of Solid State Chemistry, Ural Branch of the Russian Academy of Sciences

Yekaterinburg, Russia

S. A Brilliant

Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences;GAUZ SO “Institute for Medical Cell Technologies”

Email: svetlana.brilliant@bk.ru
Yekaterinburg, Russia

A. A Vasin

Institute of Solid State Chemistry, Ural Branch of the Russian Academy of Sciences

Yekaterinburg, Russia

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