Hepatopancreatic Proteins of Snow Crab and Red King Crab with Antibacterial Activity

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Troughout their long history, crustaceans, despite lacking a highly specific vertebrate-like adaptive immune system, have successfully adapted to living within their environment rich in microorganisms, in part due to the presence of antimicrobial peptides. One valuable source of antimicrobial peptides is hepatopancreas, a waste product from crab fishery and its processing. Data from zymogram and 1H NMR spectra showed that the extract of snow crab hepatopancreas contains a small peptide (about 3 kDa) that hydrolyzes the cell wall and the cell wall polysaccharide of M. lysodeikticus. The discovered peptide may be of interest for practical application. A protein (about 14 kDa), isolated from the red king crab hepatopancreas using affinity chromatography on heparin-Sepharose, is also active against the cell wall of the gram-positive bacterium M. lysodeikticus, as it was demonstrated with zymography and turbidimetric methods.

About the authors

V. G Molchanov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Russia

A. Y Yegorov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Russia

D. A Osetrina

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Russia

V. Yu Novikov

Polar Branch of Russian Federal Research Institute of Fisheries and Oceanography

Murmansk, Russia

N. M Novojilov

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Moscow, Russia

A. A Timchenko

Institute of Protein Research, Russian Academy of Sciences

Pushchino, Russia

E. A Sogorin

Institute for Biological Instrumentation, Russian Academy of Sciences

Pushchino, Russia

M. A Timchenko

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: maria_timchenko@mail.ru
Pushchino, Russia

References

  1. Bulet P., Stocklin R., and Menin L. Anti-microbial peptides: from invertebrates to vertebrates. Immunol. Rev., 198, 169–184 (2004). doi: 10.1111/j.0105-2896.2004.0124.x
  2. Schwab G. E., Reeves P. R., and Turner K. J. Bactericidal Activity of Serum of the Yabbie (Parachaeraps bicarinatus). Br. J. Exp. Pathol., 47 (3), 266–274 (1966). PMC2093715
  3. Stewart J. E. and Zwicker B. M. Natural and induced bactericidal activities in the hemolymph of the lobster, Homarus americanus: products of hemocyte-plasma interaction. Canad. J. Microbiol., 18 (9), 1499–1509 (1972). doi: 10.1139/m72-229
  4. Chisholm J. R. S. and Smith V. J. Antibacterial activity in the haemocytes of the shore crab, Carcinus maenas. J. Marine Biol. Association of the United Kingdom, 72 (3), 529–542 (1992). doi: 10.1017/S0025315400059324
  5. Noga E. J., Arroll T. A., Bullis R. A., and Khoo L. Antibacterial activity in hemolymph of white shrimp Penaeus setiferus. J. Mar. Biotechnol., 4 (3), 181–184 (1996).
  6. Smith V. J. and Dyrynda E. A. Antimicrobial proteins: from old proteins, new tricks. Mol. Immunol., 68 (2 Pt B), 383–398 (2015). doi: 10.1016/j.molimm.2015.08.009
  7. Ponomareva T., Timchenko M., Filippov M., Lapaev S., and Sogorin E. Prospects of red king crab hepatopancreas processing: fundamental and applied biochemistry. Recycling, 6 (1), 3 (2021). doi: 10.3390/recycling6010003T
  8. Рысакова К. С., Новиков В. Ю., Шумская Н. В. и Мухортова А. М. Выделение хитиназ из гепатопанкреаса камчатского краба и краба-стригуна опилио. В сб. Матер. 16-й Всерос. конф. «Современные перспективы в исследовании хитина и хитозана (Росхит-23)» (Изд-во Дальневосточного федерального университета, Владивосток, 2023), сс. 55–59. doi: 10.24866/7444-5553-8, EDN: YHTJSV
  9. Glyantsev S. P., Adamyan A. A., Vishnevsky A. V., and Sakharov Yu. Crab collagenase in wound debridement. J. Wound Care, 6 (1), 13–16 (1997). doi: 10.12968/jowc.1997.6.1.13, EDN: SGNGPV
  10. Haug T., Kjuul A. K., Stensvag K., Sandsdalen E., and Styrvold O. B. Antibacterial activity in four marine crustacean decapods. Fish & Shellfish Immunol., 12 (5), 371–385 (2002). doi: 10.1006/fsim.2001.0378
  11. Moe M. K., Haug T., Sydnes M. O., Sperstad S. V., Li C., Vaagsfjord L. C., de la Vega E., and Stensvag K. Paralithocins, antimicrobial peptides with unusual disulfide connectivity from the red king crab, Paralithodes camtschaticus. J. Natural Products, 81 (1), 140–150 (2018). doi: 10.1021/acs.jnatprod.7b00780
  12. Sperstad S. V., Haug T., Paulsen V., Rode T. M., Strandskog G., Solem S. T., Styrvold O. B., and Stensvag K. Characterization of crustins from the hemocytes of the spider crab, Hyas araneus, and the red king crab, Paralithodes camtschaticus. Devel. Compar. Immunol., 33 (4), 583–591 (2009). doi: 10.1016/j.dci.2008.10.010
  13. Molchanov V., Yegorov A., Molchanov M., Timchenko A., Novikov V., Novojilov N., and Timchenko M. Novel Antimicrobial peptide from the hepatopancreas of the red king crab. Int. J. Mol. Sci., 24 (21), 15607 (2023). doi: 10.3390/ijms242115607
  14. Beaulieu L., Thibodeau J., Bryl P., and Carbonneau M. E. Characterization of enzymatic hydrolyzed snow crab (Chionoecetes opilio) by-product fractions: a source of high-valued biomolecules. Bioresource Technol., 100 (13), 3332–3342 (2009). doi: 10.1016/j.biortech.2009.01.073
  15. Beaulieu L., Thibodeau J., Desbiens M., SaintLouis R., Zatylny-Gaudin C., and Thibault S. Evidence of antibacterial activities in peptide fractions originating from snow crab (Chionoecetes opilio) byproducts. Probiotics and Antimicrobial Proteins, 2 (3), 197–209 (2010). doi: 10.1007/s12602-010-9043-6
  16. El Menif E., Offret C., Labrie S., and Beaulieu L. Identification of peptides implicated in antibacterial activity of snow crab hepatopancreas hydrolysates by a bioassayguided fractionation approach combined with mass spectrometry. Probiotics and Antimicrobial Proteins, 11 (3), 1023–1033 (2019). doi: 10.1007/s12602-018-9484-x
  17. Fukutomi T., Kodera Y., Kogo T., Furudate S.-I., Omori A., and Maeda T. A simple method for peptide purification as a basis for peptidome analysis. J. Electrophoresis, 49 (1), 15–21 (2005). doi: 10.2198/jelectroph.49.15
  18. Fukushima T. and Sekiguchi J. Zymographic techniques for the analysis of bacterial cell wall in bacillus. In Methods in Molecular Biology (Clifton, N.J., 2016), vol. 1440, pp. 87–98. doi: 10.1007/978-1-4939-3676-2_7
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 (5259), 680–685 (1970). doi: 10.1038/227680a0
  20. Fales F. W., Russell J. A., and Fain J. N. Some applications and limitations of the enzymic, reducing (Somogyi), and anthrone methods for estimating sugars. Clin. Chem., 7, 389−403 (1961).
  21. Compton S. J. and Jones C. G. Mechanism of dye response and interference in the Bradford protein assay. Anal. Biochem., 151 (2), 369–374 (1985). doi: 10.1016/0003-2697(85)90190-3
  22. Destoumieux D., Bulet P., Loew D., Van Dorsselaer A., Rodriguez J., and Bachere E. Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (Decapoda). J. Biol. Chem., 272 (45), 28398–28406 (1997). doi: 10.1074/jbc.272.45.28398
  23. Stensvag K., Haug T., Sperstad S. V., Rekdal O., Indrevoll B., and Styrvold O. B. Arasin 1, a proline-argininerich antimicrobial peptide isolated from the spider crab, Hyas araneus. Devel. Compar. Immunol., 32 (3), 275–285 (2008). doi: 10.1016/j.dci.2007.06.002
  24. Sperstad S. V., Haug T., Vasskog T., and Stensvag K. Hyastatin, a glycine-rich multi-domain antimicrobial peptide isolated from the spider crab (Hyas araneus) hemocytes. Molecular Immunol., 46 (13), 2604–2612 (2009). doi: 10.1016/j.molimm.2009.05.002
  25. Noga E. J., Stone K. L., Wood A., Gordon W. L., and Robinette D. Primary structure and cellular localization of callinectin, an antimicrobial peptide from the blue crab. Devel. Compar. Immunol., 35 (4), 409–415 (2011). doi: 10.1016/j.dci.2010.11.015
  26. Kushibiki T., Kamiya M., Aizawa T., Kumaki Y., Kikukawa T., Mizuguchi M., Demura M., Kawabata S. I., and Kawano K. Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide. Biochim. Biophys. Acta −Proteins and Proteomics, 1844 (3), 527−534 (2014). doi: 10.1016/j.bbapap.2013.12.017
  27. Schnapp D., Kemp G. D., and Smith V. J. Purification and characterization of a proline-rich antibacterial peptide, with sequence similarity to bactenecin-7, from the haemocytes of the shore crab, Carcinus maenas. Eur. J. Biochem., 240 (3), 532–539 (1996). doi: 10.1111/j.1432-1033.1996.0532h.x

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Russian Academy of Sciences