Effect of Viscous Media on the Quantum Yield of Bioluminescence in a Reaction Catalyzed by Bacterial Luciferase

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Abstract

Based on previously obtained data on the transient kinetics of the bioluminescent reaction catalyzed by P. leiognathi luciferase in media with polyols and sugars, the relative quantum yield of bioluminescence per substrate molecule was determined using mathematical modeling. It was found that in some media the relative quantum yield per aldehyde molecule increases compared to the value in the buffer: by 18% in the presence of glycerol and by 33% in the presence of sucrose. The conformation of the side chain of αHis44, which is the functionally important and conservative in all bacterial luciferases, was analyzed using molecular dynamics methods. It has been established that in the presence of all cosolvents there is an increase in the probability of formation the optimal for catalysis orientation of this amino acid, which may contribute to the observed increase in the quantum yield of bioluminescence in viscous media.

About the authors

A. E Lisitsa

Siberian Federal University

Email: ALisitsa@sfu-kras.ru
Krasnoyarsk, Russia

L. A Sukovatyi

Siberian Federal University

Krasnoyarsk, Russia

V. A Kratasyuk

Siberian Federal University; Institute of Biophysics, Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences

Krasnoyarsk, Russia; Krasnoyarsk, Russia

E. V Nemtseva

Siberian Federal University; Institute of Biophysics, Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences

Krasnoyarsk, Russia; Krasnoyarsk, Russia

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