Adsorption of Proteins onto Nitrocellulose Membranes from a Flowing Solution – Theory and Experiment

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Abstract

Some analytical laboratory procedures involve passing the sample through a porous polymer membrane. In this process, the analyte binds to the surface of the membrane modified with a specific receptor layer and is then detected using optical or electrochemical signals. This paper presents an experimental and theoretical analysis of the binding patterns of the analyte to nitrocellulose membranes. Two cases are considered: specific binding of the analyte to antibodies immobilized on the membrane and non-specific adsorption of the analyte. It is shown that increasing the volume of the sample passed through the membrane leads to an increase in the amount of adsorbed analyte, and this can generally be used to improve the sensitivity of biosensors.

About the authors

K. A Prusakov

Yu.M. Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency of the Russian Federation

Moscow, 119435 Russia

S. V Zamalutdinova

M.V. Lomonosov Moscow State University

Moscow, 119991 Russia

A. E Sidorova

M.V. Lomonosov Moscow State University

Moscow, 119991 Russia

D. V Bagrov

M.V. Lomonosov Moscow State University

Email: bagrov@mail.bio.msu.ru
Moscow, 119991 Russia

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