Bioluminescent Test System Based on Recombinant L. mingrelica Firefly Luciferase as a Means of Investigating the Efficacy of Gentamicin Effect on E. coli Living Cells

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Abstract

This paper highlights the possibilities of applicability of a test system based on E. coli BL-21 Codon Plus (DE3) living cells expressing pH-tolerant thermostable Luciola mingrelica firefly luciferase to study the kinetic mechanism by which aminoglycosides, such as gentamicin used in this study, act taking into account changes in the ATP and firefly luciferase levels inside and outside the cells. It has been shown that it is possible to evaluate the changes in cell viability, assess the effectiveness of the antibiotic action, and predict the formation of persisters after incubation of bacteria with antibiotic for 3 hours. The method is promising for rapid primary high-throughput screening of antibacterial agents and dosage forms to assess their effectiveness and mechanism of action.

About the authors

G. Yu Lomakina

Department of Chemistry, M.V. Lomonosov Moscow State University

Email: lomakinagalina@yahoo.com
Moscow, Russia

S. S Kaminskaya

Department of Chemistry, M.V. Lomonosov Moscow State University; Department of Fundamental Sciences, N.E. Bauman Moscow State Technical University

Moscow, Russia; Moscow, Russia

N. N Ugarova

Department of Chemistry, M.V. Lomonosov Moscow State University

Moscow, Russia

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