The Use of the Bioluminescent Enzyme Bioassay for the Analysis of Saliva of Railway Transport Workers to Monitor the Functional State of the Body in the Conditions of Labor Activity

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Abstract

This paper proposes bioluminescent enzymatic assay of saliva as a non-invasive method for monitoring the functional state of the body. The value of luminescence intensity from coupled enzyme-based reaction catalyzed by NADH:FMN oxidoreductase and luciferase, when exposed to saliva, served as an indicator of the state of the body of workers at work. The absence of a significant difference between profiles of bioluminescence before and after the shift and no changes in biochemical, physical and chemical parameters of saliva indicated that the bodies had adapted to a workload. An increased bioluminescent intensity value is typical for workers in a state of chronic fatigue, a decreased one points to the presence of chronic diseases and bad habits. The dependence of the bioluminescent intensity value on the concentration of lactate, lipid peroxidation products, ionic and mineral composition, the values of free radical oxidation and antiradical protection was identified. Thus, the dependence of the saliva constituents on conditions of life and health status of workers can be identified using bioluminescent enzyme bioassay that is suitable for rapid monitoring of the body at work.

About the authors

L. V Stepanova

Siberian Federal University

Email: slyudmila@mail.ru
Krasnoyarsk, Russia

O. A Kolenchukova

Siberian Federal University; Scientific Research Institute of Medical Problems of the North, Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences; Krasnoyarsk State Agrarian University

Krasnoyarsk, Russia; Krasnoyarsk, Russia; Krasnoyarsk, Russia

G. V Zhukova

Siberian Federal University

Krasnoyarsk, Russia

O. S Sutormin

Siberian Federal University; Surgut State University

Krasnoyarsk, Russia; Surgut, Russia

V. A Kratasyuk

Siberian Federal University; Institute of Biophysics, Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences

Krasnoyarsk, Russia; Krasnoyarsk, Russia

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