Studying the Genetic Diversity of the Rice Break Cause  Pyricularia oryzae  Cav. on the Complex of Molecular and Morphological Features

D. V. Nartymov; Нартымов Д. В.; E. V. Dubina; Дубина Е. В.; M. G. Ruban; Рубан М. Г.; Yu. V. Aniskina; Анискина Ю. В.; S. V. Garkusha; Гаркуша С. В.; I. A. Shilov; Шилов И. А.; N. S. Velishaeva; Велишаева Н. С.; O. S. Kolobova; Колобова О. С.; N. K. Istomin; Истомин Н. К.

doi:10.31857/S0016675823060127

Studying the Genetic Diversity of the Rice Break Cause Pyricularia oryzae Cav. on the Complex of Molecular and Morphological Features

Authors: Nartymov D.V.¹, Dubina E.V.¹^,2, Ruban M.G.¹, Aniskina Y.V.³, Garkusha S.V.¹^,2, Shilov I.A.³, Velishaeva N.S.³, Kolobova O.S.³, Istomin N.K.¹
Affiliations:
1. Federal Scientific Rice Centre
2. Trubilin Kuban State Agrarian University
3. All-Russian Research Institute of Agricultural Biotechnology
Issue: Vol 59, No 6 (2023)
Pages: 640-647
Section: ГЕНЕТИКА МИКРООРГАНИЗМОВ
URL: https://ruspoj.com/0016-6758/article/view/666840
DOI: https://doi.org/10.31857/S0016675823060127
EDN: https://elibrary.ru/SUHFYL
ID: 666840

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Abstract

On the basis of molecular genetic approaches, the study of the biodiversity of the phytopathogenic fungus Pyricularia oryzae Cav. in the south of Russia and a classification of the morphological and cultural characteristics of the pathogen was carried out. For 2020–2022 monitoring was carried out and 24 strains of the pathogen were isolated from the affected herbarium material collected from the fields of five ecological rice growing zones of the Krasnodar Territory (Krasnoarmeisky, Kalininsky, Abinsky, Slavyansky districts, Krasnodar). Using the multiplex PCR system developed by us based on fragment analysis, five genotypes were identified among the studied Pyricularia oryzae Cav. strains over the entire period of the project, each of which is characterized by a unique genetic profile. Compiled their “DNA passport”. The studied isolates of the causative agent of blast were characterized by morphological and cultural characteristics. Six morphotypes of the pathogen have been identified.

Keywords

genetic biodiversity, phytopathogenic fungi, Pyricularia oryzae Cav. (blast), pathotype, strains, SSR loci, PCR, DNA passports.

References

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