Analysis of  ADCY8  и  RYR3  Genes Polymorphism for Identification Wild and Domestic Animal of Species  Canis lupus

V. N. Kipen; Кипень В. Н.; M. M. Patrin; Патрин М. М.; E. V. Snytkov; Снытков Е. В.; A. N. Viarchuk; Верчук А. Н.; A. N. Semak; Семак А. Н.

doi:10.31857/S0016675823030062

Analysis of ADCY8 и RYR3 Genes Polymorphism for Identification Wild and Domestic Animal of Species Canis lupus

Authors: Kipen V.N.¹, Patrin M.M.², Snytkov E.V.¹^,3, Viarchuk A.N.¹^,4, Semak A.N.⁵
Affiliations:
1. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
2. Maxim Medical LLC
3. International Sakharov Environmental Institute of Belarusian State University
4. Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus
5. WellVet LLC
Issue: Vol 59, No 3 (2023)
Pages: 336-344
Section: ГЕНЕТИКА ЖИВОТНЫХ
URL: https://ruspoj.com/0016-6758/article/view/666878
DOI: https://doi.org/10.31857/S0016675823030062
EDN: https://elibrary.ru/IOHKSC
ID: 666878

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription or Fee Access

Abstract

As a result of the study, we confirmed the high differentiating potential of polymorphic variants g.27748425T>C (ADCY8 gene) and g.1414373T>C (RYR3 gene). A test model of two polymorphisms for the differentiation of a wolf and a domestic dog is proposed, which is distinguished by high values of accuracy (96.2%), specificity (96.3%) and sensitivity (98.9%). Using KASP, a quick and simple approach to differentiation based on the proposed test model has been developed, which is designed to reduce the time and cost of molecular genetic analysis, as well as reduce the risk of cross-contamination, because the process is one-stage (restriction and electrophoresis stages are excluded).

Keywords

Canis lupus, Canis lupus familiaris, SNP, differentiation, kompetitive allele-specific PCR (KASP), RFLP-PCR, high-resolution amplicon melting (HRM), in silico genotyping.

References

Hindrikson M., Remm J., Pilot M. et al. Wolf population genetics in Europe: A systematic review, meta-analysis and suggestions for conservation and management // Biol. Rev. 2016. V. 92(3). P. 1601–1629. https://doi.org/10.1111/brv.12298
Cronin M.A., Canovas A., Bannasch D.L. et al. Single nucleotide polymorphism (SNP) variation of wolves (Canis lupus) in Southeast Alaska and comparison with wolves, dogs, and coyotes in North America // J. Hered. 2015. V. 106(1). P. 26–36. https://doi.org/10.1093/jhered/esu075
Vonholdt B.M., Pollinger J.P., Lohmueller K.E. et al. Genome-wide SNP and haplotype analyses reveal a rich history underlying dog domestication // Nature. 2010. V. 464(7290). P. 898–902. https://doi.org/10.1038/nature08837
Гребенчук А.Е. Псовые как объект экспертного ДНК-анализа: криминалистические и генетические аспекты // Вопр. криминологии, криминалистики и судебной экспертизы. 2016. № 2 (40). С. 135–140.
Хейдорова Е.Э., Шпак А.В., Гомель К.В. и др. Молекулярно-генетическая идентификация инвазивного вида – шакала азиатского (Canis aureus) на территории Беларуси // Докл. НАН Беларуси. 2018. Т. 62. № 1. С. 86–92. https://doi.org/10.29235/1561-8323-2018-62-1-86-92
Кипень В.Н., Иванова Е.В., Снытков Е.В., Верчук А.Н. Анализ полиморфизма гена гефестина (HEPH) на Х-хромосоме для установления принадлежности биологических образцов к диким или домашним представителям вида Sus scrofa // Генетика. 2020. Т. 56. № 9. С. 1054–1064. https://doi.org/10.31857/S0016675820080068
Кипень В.Н., Михалова М.Е., Снытков Е.В. и др. Биоинформатический анализ геномов коммерческих пород домашних свиней для идентификации породоспецифичных SNP // Весці Нац. акад. навук Беларусі. Серыя аграрных навук. 2021. Т. 59. № 4. С. 464–476. https://doi.org/10.29235/1817-7204-2021-59-4-464-476
Ramos A.M. Identification of high utility SNPs for population assignment and traceability purposes in the pig using high-throughput sequencing // Anim. Genet. 2011. V. 42(6). P. 613–620. https://doi.org/10.1111/j.1365-2052.2011.02198.x
Okonechnikov K., Golosova O., Fursov M. Unipro UGENE: A unified bioinformatics toolkit // Bioinformatics. 2012. V. 28. P. 1166–1167. https://doi.org/10.1093/bioinformatics/bts091
Кипень В.Н., Снытков Е.В., Кривенко А.А., Патрин М.М. In silico анализ полиморфизма H3GA0051811 гена HEPH для животных вида Sus scrofa // VII Междунар. конф. молодых ученых: биофизиков, биотехнологов, молекулярных биологов и вирусологов в рамках площадки открытых коммуникаций OpenBio-2020. Новосибирск: 2020. С. 469–472.
Ritchie M.D., Hahn L.W., Roodi N. et al. Multifactor-dimensionality reduction reveals high-order interactions among estrogen-metabolism genes in sporadic breast cancer // Am. J. Hum. Genet. 2001. V. 69(1). P. 138–147. https://doi.org/10.1086/321276