Аnalyzing buckwheat genotypes obtained on selective media with zinc in vitro using ISSR markers

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

The paper evaluates four ISSR-markers (М1, М2, М7, and М11) for their effectiveness in research on the genetic distances of regenerated Fagopyrum esculentum plants, which were obtained in vitro on selective media with high doses of ZnSO4×7 H2O (808–1313 mg/L). The level of polymorphism was determined to be high in the combined sample (74.4%). The obtained speciemens might be used in the breeding of new varieties with economically important traits.

Full Text

Restricted Access

About the authors

S. A. Borovaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Author for correspondence.
Email: borovayasveta@mail.ru
Russian Federation, Primorsky Krai, Timiriasevsky, 692539

A. G. Klykov

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
Russian Federation, Primorsky Krai, Timiriasevsky, 692539

N. G. Boginskaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
Russian Federation, Primorsky Krai, Timiriasevsky, 692539

References

  1. Chrungoo N.K., Dohtdong L., Chettry U. Phenotypic рlasticity in buckwheat // Mol. Breeding and Nutritional Aspects of Buckwheat. London: Elsevier, Academic Press, 2016. Р. 137–149. https://doi.org/10.1016/B978-0-12-803692-1.00010-9
  2. Blair M., Panaud O., McCouch S. Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.) // Theor. Appl. Genetics. 1999. V. 98. P. 780–792. https://doi.org/10.1007/s001220051135
  3. Gilbert J.E., Lewis R.V., Wilkinson M.J., Caligari P.D.S. Developing an appropriate strategy to assess genetic variability in plant germplasm collections // Theor. Appl. Genetics. 1999. V. 98. № 6–7. P. 1125–1131. https://doi.org/10.1007/s001220051176
  4. Sica M., Gamba G., Montieri S., Gaudio L., Aceto S. ISSR markers show differentiation among Italian populations of Asparagus acutifolius // BMC Genetics. 2005. V. 6. P. 1–7. https://doi.org/10.1186/1471-2156-6-17
  5. Meloni M., Perini D., Filigheddu R., Binelli G. Genetic variation in five Mediterranean populations of Juniperus phoenicea as revealed by inter-simple sequence repeat (ISSR) markers // Ann. Bot. 2006. V. 97. № 2. P. 299–304. https://doi.org/10.1093/aob/mcj024
  6. Alhasnawi A.N., Kadhimi А.А., Isahak А. et al. Application of inter simple sequence repeat (ISSR) for detecting genetic analysis in rice (Oryza sativa L.) // J. Pure Appl. Microbiology. 2015. V. 9. № 2. P. 1091–11016.
  7. Aldaej M.I., Rezk A.A., El-Malky M., Shalaby T.A. Comparative genetic diversity assessment and Marker-Trait Association using two DNA marker systems in rice (Oryza sativa L.) // Agronomy. 2023. V. 13. № 2. https://doi.org/10.3390/agronomy13020329
  8. Кадырова Г.Д., Кадырова Ф.З., Мартиросян Е.В., Рыжова Н.Н. Анализ геномного разнообразия образцов и сортов гречихи посевной и татарской ISSR-методом // С.-хоз. биология. 2010. Т. 5. С. 42–48.
  9. Клыков А.Г., Барсукова Е.Н. Биотехнология и селекция гречихи на Дальнем Востоке России. Владивосток: ООО “ПСП95”, 2021. 352 с.
  10. Sabreena N. М., Mahajan R., Hashim M.J. et al. Deciphering allelic variability and population structure in buckwheat: An analogy between the efficiency of ISSR and SSR markers // Saudi J. Biol. Sci. 2021. V. 28. Р. 6050–6056. https://doi.org/10.1016/j.sjbs.2021.07.061
  11. Shukla A., Srivastava N., Suneja P. et al. Genetic diversity analysis in Buckwheat germplasm for nutritional traits // Indian J. Experim. Biology. 2018. V. 56. № 11. Р. 827–837.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. UPGMA is a dendrogram based on the values of genetic distances for buckwheat regenerants. 1 is a group of variants characterized by the smallest DN compared to the original form; 2 is a group of variants characterized by the largest DN compared to the original form.

Download (1KB)
Indexing metadata

Copyright (c) 2024 Russian Academy of Sciences