Regulation of Uni-Strand and Dual-Strand piRNA Clusters in Germ and Somatic Tissues in Drosophila melanogaster under Control of rhino

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Abstract

Drosophila melanogaster is a common genetic object for research of RNA-interference pathways and mobile elements regulation. Nowadays taking a part in control of retrotransposon expression the system of piRNA-interfecence well studied in ovary tissues. It is strongly believed that D. melanogaster piRNA-interference is used for retrotransposon suppression only in gonads, and two distinct pathways of piRNA biogenesis exist. Both mechanisms use transcripts of piRNA-clusters (accumulations of truncated and defect mobile elements copies): from unstrand clusters in the first case and from dualstrand clusters in the second, transcribed with one or both DNA chains correspondingly. It is well-known that proper dualstrand clusters function depends on the gene rhino, while unistrand clusters are transcribed rhino-independent and transcripts are spliced. In this paper we show that rhino participates in unistrand flamenco transcripts splicing and the piRNA-interference significance for regulation of several retrotransposons not only in gonads, but in other organs.

About the authors

P. A. Milyaeva

Lomonosov Moscow State University; Faculty of Biology, Shenzhen MSU-BIT University

Email: nefedova@mail.bio.msu.ru
Russia, 119234, Moscow; China, 518172, Longgang District, Shenzhen

A. R. Lavrenov

Lomonosov Moscow State University; Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences

Email: nefedova@mail.bio.msu.ru
Russia, 119234, Moscow; Russia, 119071, Moscow

I. V. Kuzmin

Lomonosov Moscow State University

Email: nefedova@mail.bio.msu.ru
Russia, 119234, Moscow

A. I. Kim

Lomonosov Moscow State University; Faculty of Biology, Shenzhen MSU-BIT University

Email: nefedova@mail.bio.msu.ru
Russia, 119234, Moscow; China, 518172, Longgang District, Shenzhen

L. N. Nefedova

Lomonosov Moscow State University

Author for correspondence.
Email: nefedova@mail.bio.msu.ru
Russia, 119234, Moscow

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Copyright (c) 2023 П.А. Миляева, А.Р. Лавренов, И.В. Кузьмин, А.И. Ким, Л.Н. Нефедова