Molecular Marking in  Brassica oleracea  L. Breeding for Resistance to Fusarium Wilt

E. V. Dubina; Дубина Е. В.; Yu. A. Makukha; Макуха Ю. А.; A. M. Artem’eva; Артемьева А. М.; D. A. Fateev; Фатеев Д. А.; S. V. Garkusha; Гаркуша С. В.; O. L. Gorun; Горун О. Л.; S. A. Lesnyak; Лесняк С. А.

doi:10.31857/S001667582310003X

Molecular Marking in Brassica oleracea L. Breeding for Resistance to Fusarium Wilt

Authors: Dubina E.V.¹^,2, Makukha Y.A.¹, Artem’eva A.M.³, Fateev D.A.³, Garkusha S.V.¹, Gorun O.L.¹, Lesnyak S.A.¹
Affiliations:
1. Federal Scientific Rice Centre
2. Kuban State Agrarian University
3. Federal Research Centre Vavilov All-Russian Institute of Plant Genetic Resources
Issue: Vol 59, No 10 (2023)
Pages: 1134-1141
Section: ГЕНЕТИКА РАСТЕНИЙ
URL: https://ruspoj.com/0016-6758/article/view/667061
DOI: https://doi.org/10.31857/S001667582310003X
EDN: https://elibrary.ru/ZTYMZI
ID: 667061

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Abstract

This article presents the results of research on the identification of informative DNA marker systems, providing reliable control of the resistance gene to fusarium wilt Foc1 presence in white cabbage breeding material. At the beginning of the work 14 molecular markers, taken from VegMarks database and literature sources were tested on isogenic white cabbage lines with contrasting resistance to fusarium wilt (resistant line DT-46 and susceptible line Kb1P). InDel-marker M10 and SSR-markers Frg13 and O110-D01 have been ascertained to show polymorphism between white cabbage forms with contrasting resistance to fusarium wilt. Also PCR-analysis of segregating F₂-population plants of hybrid combination DT-46 × Kb1P using these markers and phytopathology testing have been conducted. As a result of statistical analysis of segregation it has been found that SSR-marker Ol10-D01 only is cosegregated with the trait of resistance to fusarium wilt as expected segregation of F₂-plants by genotype 1 : 2 : 1 according to Mendel’s law, the least recombination frequency between the resistance gene Foc1 and the marker (1.6%) has just been observed by this locus only and highly polymorphic (PIC = 0.51).

Keywords

white cabbage, fusarium wilt, SSR-marker, PCR-analysis, segregating population.

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