The genome of Staphylococcus epidermidis Isolated from Caseous Tuberculoma

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Abstract

A number of facultative-anaerobic lipophilic microorganisms, including representatives of Corynebacterium and Staphylococcaceae, inhabit the necrotic contents of tuberculomas. A strain of Staphylococcus epidermidis was isolated from caseum, fully genome sequenced, and gene-mapped. The coagulase-negative Staphylococcus belonged to the MLST 73 genotype and was resistant to two antituberculosis drugs. The strain phenotypically had urease, gelatinase and beta-hemolytic activities and possessed the corresponding genes. Similar to S. epidermidis O47, its genome consists of a single chromosome containing approximately 2.4 million base pairs, and oriC has the same orientation. A total of 2333 genes were identified, of which 2206 were coding genes. In contigs of the genome, sequences of plasmid replication genes were found: rep7a, rep13, rep5b and pSK1. Phylogenetic analysis indicates the closeness of the analyzed genome with a large group of European strains. Considering the biochemical and microbiological properties of the isolated strain, we hypothesize that staphylococci and other facultative-anaerobic satellite microorganisms of tuberculosis foci may play an important role in caseum liquefaction due to their own proteolytic activity and attraction of neutrophils to the focus of inflammation.

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About the authors

V. V. Sinkov

Scientific Сentre for Family Health and Human Reproduction Problems

Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003

E. A. Orlova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003

O. B. Ogarkov

Scientific Сentre for Family Health and Human Reproduction Problems

Author for correspondence.
Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003

A. E. Suzdalnitsky

Irkutsk Tuberculosum-prevention hospital; Irkutsk State Medical University

Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664039; Irkutsk, 664003

I. G. Kondratov

Scientific Сentre for Family Health and Human Reproduction Problems

Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003

N. L. Belkova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003

L. V. Rychkova

Scientific Сentre for Family Health and Human Reproduction Problems

Email: obogarkov@mail.ru
Russian Federation, Irkutsk, 664003

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2. Fig. 1. Maximum Parsimony (MP) – phylogenetic tree with bootstrap analysis. The background is the genome of the studied strain and the cluster of S. epidermidis genomes from Europe (BioProject PRJEB31403), to which the strain isolated in this study belongs.

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