Nematode Caenorhabditis elegans as an object for testing the genotoxicity of chemical compounds

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Abstract

In connection with the requirements of International and national organizations to comply with the principles of humanization of experiments using animals, alternative test systems are being searched and tested to replace animals in ecological, toxicological and genotoxicological studies. One such alternative may be the nematode Caenorhabditis elegans, which has a biotransformation system of chemical compounds similar to the mammalian system. The genotoxicity of the pesticides paraquat and the antibacterial agent furacilin was studied on C. elegans by horizontal gel electrophoresis of total DNA in order to assess its integrity. It has been shown that paraquat in concentrations of 0.01 to 0.05 mol/l and furacilin f in concentrations of 0.0001 and 0.00025 mol/l, caused DNA breaks in nematode cells. The antioxidant N-acetylcysteine at a concentration of 0.01 mol/l reduced the genotoxicity of both compounds.

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About the authors

S. K. Abilev

Vavilov Institute of General Genetics Russian Academy of Science

Author for correspondence.
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991

E. M. Machigov

Vavilov Institute of General Genetics Russian Academy of Science

Email: abilev@vigg.ru
Russian Federation, Moscow, 119991

S. V. Smirnova

Vavilov Institute of General Genetics Russian Academy of Science

Email: abilev@vigg.ru
Russian Federation, Moscow, 119991

M. V. Marsova

Vavilov Institute of General Genetics Russian Academy of Science

Email: abilev@vigg.ru
Russian Federation, Moscow, 119991

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2. Fig. 1. Results of gel electrophoresis of the genetic material of the nematode C. elegans exposed to paraquat at different concentrations (mol/l). 1 – marker, 2 – water (control), 3 – 0.0005, 4 – 0.001, 5 – 0.0025, 6 – 0.005, 7 – 0.01, 8 – 0.025, 9 – 0.05, 10 – hydrogen peroxide 0.1 mol/l (positive control).

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3. Fig. 2. Results of gel electrophoresis of the genetic material of the nematode C. elegans exposed to paraquat at a concentration of 0.05 and ACC at concentrations of 0.01 and 0.001 mol/l: 1 – marker, 2 – water (negative control), 3 – paraquat 0.05 + ACC 0.01, 4 – paraquat 0.05 + ACC 0.001, 5 – paraquat 0.05 mol/l.

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4. Fig. 3. Results of gel electrophoresis of the genetic material of the nematode C. elegans exposed to furacilin at different concentrations (mol/l): 1 – marker, 2 – water (control), 3 – 0.000005, 4 – 0.00001, 5 – 0.00002, 6 – 0.00005, 7 – 0.0001, 8 – 0.00025; positive controls: 9 – BPL 0.015 mol/l and 10 – hydrogen peroxide 0.1 mol/l.

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5. Fig. 4. Results of gel electrophoresis of the genetic material of the nematode C. elegans exposed to furacilin at a concentration of 0.00025 and ACC at concentrations of 0.01 and 0.001 mol/l: 1 – marker, 2 – water (control), 3 – furacilin 0.00025 + ACC 0.01, 4 – furacilin 0.00025 + ACC 0.001, 5 – furacilin 0.00025 mol/l.

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