Assessing the Genetic Diversity of Five Cattle Breeds Using SNP Markers Associated with Health

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Abstract

Currently genetic evaluation of animals is an important part of the development of the agricultural complex. The improvement of molecular technologies every year makes it possible to carry out genetic research aimed at finding the most valuable animals in a cheaper and faster way. Indigenous breeds of cattle are an attractive object for such research because they have greater adaptive potential and resistance to diseases. However, modern comparative data on the genetic diversity of most local breeds based on SNP markers associated with health are lacking. Genetic association tests using these genetic markers for the Tagil, Sychevskaya, Suksun and Istobenskaya breeds are still to be carried out. The purpose of this work was to compare the genetic diversity of five cattle breeds using SNP markers associated with the development of ketosis, mastitis and productive longevity.

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About the authors

M. V. Bytov

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

V. D. Zubareva

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

S. V. Volskaya

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

A. G. Isaeva

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

D. Yu. Nokhrin

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

Yu. A. Osipova

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

O. V. Sokolova

Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences

Author for correspondence.
Email: nauka_sokolova@mail.ru
Russian Federation, Ekaterinburg, 620142

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2. Fig. 1. Deviations of the observed genotype frequencies from those expected by HardyTo Weinberg for two studied breeds of dairy cattle according to rs109452259. The spread on the histogram indicates 95% CI based on the results of Monte Carlo simulation in the HW_TEST package.

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3. Fig. 2. Results of capillary electrophoresis of amplification products obtained using the developed TaqMan genotyping systems.

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4. Fig. 3. The ratio of the frequencies of the genotypes of the three polymorphisms in the Ural populations of dairy cattle on the de Finetti diagrams. The red parabola is the Hardy-Weinberg equilibrium ratio, the red labels are populations with a deviation from equilibrium.

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5. Fig. 4. The scheme of the model of genetic cleavage of the five studied cattle breeds according to the principle of the main coordinates.

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6. Fig. 5. The results of the evaluation of the nonequilibrium coupling of the studied polymorphisms for the Istobenskaya and Tagil cattle breeds.

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