Genotoxic effect of selenium arabinogalactan nanocomposite on nucleated blood cells

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Abstract

Introduction. Selenium (Se) nanoparticles have attracted the interest of researchers for various applications due to their unusual properties. Despite their advantages, Se nanoparticles also have toxic effects, so for their successful use it is necessary to know the doses that are safe for the use. An important component in the development of pathological processes is the occurrence of DNA damage after exposure to Se nanoparticles, which can lead to severe disorders.

Materials and methods. Male white rats were orally administered a solution of Se nanocomposite at a dose of 500 µg/kg for 10 days. The genotoxicity of the nanocomposite under study was assessed by the occurrence of DNA damage in blood cells using the DNA comet method in the alkaline version. The results were obtained during 2 stages: one day after exposure and after 4 months to identify the persistence or absence of a negative effect.

Results. With using the DNA comet method, intragastric administration of Se nanocomposite was found to cause the damage to the DNA structure, and this effect persists not only 24 hours after exposure, but also 4 months later.

Limitations. The study is limited to the study of DNA fragmentation on the next day after a 10-day exposure to Se nanocomposite in male white rats and during the long-term period after 4 months.

Conclusion. The study revealed persistent DNA damage in the nucleated blood cells of male albino rats, which apparently may be associated with the main mechanism of Se toxicity: nonspecific replacement of sulfur in sulfur-containing amino acids. However, the toxic effects of the nanocomposite may also be caused by its pro-oxidant properties, which requires further confirmation.

Compliance with ethical standards. The study was approved by the local Ethics Committee of the East-Siberian Institute of Medical and Ecological Research (Protocol No.1 of 12/18/2017), conducted in accordance with the European Convention for the Protection of Vertebrates Used for Experiments or Other Scientific Purposes (ETS N 123), directive of the European Parliament and the Council Of the European Union 2010/63/EC of 22.09.2010 on the protection of animals used for scientific purposes.

Contribution:
Tyutrina V.A. — literature search, experiment, writing, statistical processing, article design;
Sosedova L.M. — concept, writing, discussion of relevance and results;
Titov E.A. — concept, experiment.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgment. The work was carried out according to the research plan within the framework of the state task.

Received: March 13, 2024 / Revised: April 8, 2024 / Accepted: June 19, 2024 / Published: July 17, 2024

 

About the authors

Vera A. Tyutrina

East-Siberian Institute of Medical and Ecological Research

Author for correspondence.
Email: tyutrina.v.a@yandex.ru
ORCID iD: 0000-0002-9406-5424

MD, PhD (Pharm), researcher at the Laboratory of biomodelling and translational medicine of the East-Siberian Institute of Medical and Ecological Research, Angarsk, 665826, Russian Federation

e-mail: tyutrina.v.a@yandex.ru

Russian Federation

Larisa M. Sosedova

East-Siberian Institute of Medical and Ecological Research

Email: sosedlar@mail.ru
ORCID iD: 0000-0003-1052-4601

MD, PhD, DSci (Med), Professor, Head of the Laboratory of biomodelling and translational medicine of the East-Siberian Institute of Medical and Ecological Research, Angarsk, 665826, Russian Federation

e-mail: sosedlar@mail.ru

Russian Federation

Evgeniy A. Titov

East-Siberian Institute of Medical and Ecological Research

Email: g57097@yandex.ru
ORCID iD: 0000-0002-0665-8060

MD, PhD (Biol), senior researcher, Laboratory of biomodelling and translational medicine of the East-Siberian Institute of Medical and Ecological Research, Angarsk, 665826, Russian Federation

e-mail: g57097@yandex.ru

Russian Federation

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