Effect of chronic stress on the degree of acrylamide toxicity in rats

Introduction. Chronic stress plays an important role in the development of a wide range of mental, somatic and behavioural disorders, and therefore is a risk factor for the health in living organisms. A negative effect on the state of the nervous system is also observed when the body is poisoned with acrylamide, which is a substance of the second class of danger and is considered toxic and carcinogenic. Exposure of the body to mental and toxic stressors leads to autonomic and neuroendocrine activation, which, in turn, manifests itself as special behavioural patterns.

The purpose of the experiment was to assess the effect of chronic stress on the degree of acrylamide toxicity in rats.

Materials and methods. The experiment was conducted on white outbred rats with an average weight of 200 g of both sexes (n=60), which were evenly distributed into five groups: negative control, chronic stress, acrylamide, acrylamide + treatment, acrylamide + chronic stress. Throughout the study, the animals were kept under standard conditions with 12 hours of artificial lighting during the day, a relatively constant level of humidity (30–70%) and an air temperature of 20–25 °C. Behavioural tests were conducted once a week for one calendar month using a 40×40 hole board, a multifunctional cage to assess general activity, and an elevated plus maze with an ANY-maze video tracking system. Biochemical parameters were assessed in accordance with the manufacturer’s instructions.

Results. Analysis of behaviour on a board with holes did not reveal statistically significant results (H=8.987; p=0.061). When comparing vertical and horizontal motor activity between groups, statistically significant differences were found (p<0.05). AST levels were higher in the stress groups, while cholesterol levels in the same groups were lower (p<0.05).

Limitations. Laboratory animals of only one biological species were used for the experiment. The toxicant was used only in one concentration.

Conclusion. Chronic stress may influence on acrylamide toxicity in rats to a certain extent.

Compliance with ethical standards. Date of the meeting of the bioethical commission of the Federal Budgetary Institution “Ufa Research Institute of Occupational Medicine and Human Ecology” 08.02.2024, No. 01-02. Manipulations with all animals were carried out strictly in compliance with the rules prescribed in basic regulatory documents, including the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986) and the Declaration of Helsinki on the Humane Treatment of Animals.

Contribution:
Gizatullina A.A. — concept and design of the study, collection and processing of material, statistical processing, text writing;
Khusnutdinova N.Yu. — collection and processing of material;
Karimov D.D. — collection and processing of material;
Smolyankin D.O. — collection and processing of material;
Valova Y.V. — collection and processing of material, statistical processing;
Karimov D.O. — concept and design of the study, statistical processing;
Muhammadieva G.F. — editing;
Repina E.F. — concept and design of the study;
Akhmadeev A.R. — collection and processing of material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. Industry research program of Rospotrebnadzor for 2021-2025 “Scientific substantiation of the national system for ensuring sanitary and epidemiological well-being, managing health risks and improving the quality of life of the population of Russia”, on the topic: “Studying the impact of chemical production factors in conditions of chronic stress” No. NIOKTR I124021200153 -3.

Received: February 20, 2024 / Accepted: March 11 2024 / Published: April 10, 2024