Experimental study of the gonadotoxic effect of forest fire smoke

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Abstract

Introduction. The study of the effects of air smoke during natural fires on the reproductive system is the actual scientific problem at present.

Material and methods. The experimental study took place in 2 stages. At the first stage of the study, white male rats were exposed to the smoke-bearing effects of wildfire for 7 days (4 hours a day), and at the second stage, 5 days a week (4 hours a day) for one month. Immediately after the end of the exposure, the animals were decapitated under light ether anesthesia to study the morphofunctional state of the reproductive system, which included counting the total number of spermatogonia, the number of tubules with desquamated epithelium, as well as determining the spermatogenesis index and the number of Leydig cells.

Results. The functional state of the reproductive system of male white rats after exposure to smoke for 1 week did not have statistically significant differences from the animals of the control group. At the same time, an increment in the duration of exposure (4 weeks) led to significant morphological and functional disorders of the reproductive system of male white rats, characterized by a decrease in the spermatogenesis index with a simultaneous decrease in the percentage of spermatogonia and the number of interstitial Leydig cells.

Discussion. Male reproductive health can be a sensitive marker of environmental factors, and there is no evidence of smoke from natural fires in modern literature. This study justifies the need to develop new approaches to the diagnosis and prevention of adverse effects on the body for a long stay in a smoke area.

Conclusion. The results of the study indicate the prolonged exposure to combustion products released during a forest fire to lead to inhibition of reproductive function in male white rats.

About the authors

Mikhail A. Novikov

East-Siberian Institute of Medical and Ecological Research

Author for correspondence.
Email: novik-imt@mail.ru
ORCID iD: 0000-0002-6100-6292

MD, Ph.D., researcher of the Laboratory of biomodeling and translational medicine, East-Siberian Institute of Medical and Ecological Research, Angarsk, 665827, Russian Federation.

e-mail: novik-imt@mail.ru

Russian Federation

Vera A. Vokina

East-Siberian Institute of Medical and Ecological Research

Email: noemail@neicon.ru
ORCID iD: 0000-0002-8165-8052
Russian Federation

Elizaveta S. Andreeva

East-Siberian Institute of Medical and Ecological Research

Email: noemail@neicon.ru
ORCID iD: 0000-0002-3709-8676
Russian Federation

Anton N. Alekseenko

East-Siberian Institute of Medical and Ecological Research

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4980-5304
Russian Federation

Larisa M. Sosedova

East-Siberian Institute of Medical and Ecological Research

Email: noemail@neicon.ru
ORCID iD: 0000-0003-1052-4601
Russian Federation

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Copyright (c) 2024 Novikov M.A., Vokina V.A., Andreeva E.S., Alekseenko A.N., Sosedova L.M.


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