Morphological changes in the liver parenchyma in rats under subacute acrylamide intoxication and the possibility of their preventive treatment

Introduction. Acrylamide, used in industry, is also formed in foods during high temperature processing. Acrylamide has a toxic effect on the nervous, reproductive, and other systems of the body, due to carcinogenic and genotoxic properties. Considering sufficiently serious threat of acrylamide to human health, it is relevant to carry out further fundamental research to study the mechanism of its impact on the body and the possibility of treatment of lesions caused by it.

The aims of this study is to assess morphological changes in the liver of experimental animals under acute acrylamide intoxication and study the possibility of their preventive treatment with complex compounds based on oxymethyluracil.

Materials and methods. Experimental studies on the subacute effects of acrylamide at a dose of 20 mg/kg on the histological structure of the liver were carried out on outbred female rats. The efficiency of preventive administration of complex compounds of oxymethyluracil with ascorbic acid, with sodium succinate and acetylcysteine ​​was also studied.

Results. Exposure to acrylamide for 28 days at a dose of 20 mg/kg of body weight in experimental rats, has been established to cause minor morphological changes in the structure of the liver: only focal cellular infiltrates were found in the centrilobular zones. In groups of rats that received preventive treatment with complex compounds based on oxymethyluracil, no formation of infiltrates and other pathological changes was observed.

Limitations. Limitations of the study are related to implementation of experimental studies of the pathomorphological changes in the liver of experimental animals using only one dose of acrylamide - 20 mg per kg of body weight during one limited period (28 days).

Conclusion. Exposure to acrylamide for 28 days at a dose of 20 mg/kg of body weight causes minor morphological changes in the structure of the liver in experimental rats. For the first time, the effectiveness of prophylactic correction of the toxic effect of acrylamide with complex compounds of oxymethyluracil with ascorbic acid, sodium succinate and acetylcysteine, which showed a certain protective effect on the liver, was studied.

Compliance with ethical standards. The study was approved by the Bioethical Commission of the Ufa Research Institute of Occupational Medicine and Human Ecology, conducted in accordance with the European Convention for the Protection of Vertebrate Animals Used for Experiments or for Other Scientific Purposes (ETS N 123), Directive of the European Parliament and the Council of the Eurpean Union 2010/63/EC of 22.09.2010 on the protection of animals used for scientific purposes.

Contribution:
Baygildin S.S. — conducting histological studies, writing a text;
Repina E.F. — study design, text writing;
Karimov D.O., Bakirov A.B. — study concept and design, editing;
Gimadieva A.R. — synthesis of 5-hydroxy-6-methyluracil with ascorbic acid, sodium succinate and acetylcysteine;
Khusnutdinova N.Yu., Timasheva G.V., Akhmadeev A.R., Smolyankin D.A. — data collection and processing, statistical analysis.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The work was carried out as part of the implementation of the state task for the industry research program of Rospotrebnadzor “Scientific justification of the national system for ensuring sanitary and epidemiological well-being, managing health risks and improving the quality of life of the population of Russia” for 2021-2025. clause 6.1.8, state no. registration 121062100058-8.

The synthesis of complex compounds of 5-hydroxy-6-methyluracil with ascorbic acid, sodium succinate, and acetylcysteine was carried out in accordance with the research plan of the Ufa Institute of Chemistry of the Ural Federal Research Center of the Russian Academy of Sciences (State Registration No. AAAA-A19-119011790021-4).

Received: April 12, 2023 / Accepted: June 7, 2023 / Published: July 30, 2023