An experimental trial of bioprophylactic formula designed to minimize combined toxicity of both lead and cadmium

Larisa I. Privalova; Привалова Лариса Ивановна; S. V. Klinova; Клинова С. В.; I. A. Minigalieva; Минигалиева И. А.; Iu. V. Ryabova; Рябова Ю. В.; M. P. Sutunkova; Сутункова М. П.; O. G. Makeev; Макеев О. Г.; I. E. Valamina; Валамина И. Е.; T. V. Bushueva; Бушуева Т. В.; S. N. Solovyeva; Соловьёва С. Н.; V. B. Gurvich; Гурвич В. Б.; B. A. Katsnelson; Кацнельсон Б. А.

doi:10.47470/0016-9900-2020-99-1-85-89

An experimental trial of bioprophylactic formula designed to minimize combined toxicity of both lead and cadmium

Authors: Privalova L.I.¹, Klinova S.V.¹, Minigalieva I.A.¹, Ryabova I.V.¹, Sutunkova M.P.¹, Makeev O.G.², Valamina I.E.², Bushueva T.V.¹, Solovyeva S.N.¹, Gurvich V.B.¹, Katsnelson B.A.¹
Affiliations:
1. Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
2. Central Research Laboratory of the Ural Medical University
Issue: Vol 99, No 1 (2020)
Pages: 85-89
Section: EXPERIMENTAL INVESTIGATIONS
Published: 30.01.2020
URL: https://ruspoj.com/0016-9900/article/view/640117
DOI: https://doi.org/10.47470/0016-9900-2020-99-1-85-89
ID: 640117

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Abstract

Introduction. The increase in the body resistance to the combined effects of lead and cadmium (including systemic toxicity, cardiovascular effects, and genotoxicity) by using a specific bioprotective formula (based on theoretical knowledge and experimental research) remains a pressing challenge. However, a data search has not yielded any results on either an experimental trial or a theoretical justification of the means of biological protection against a variety of adverse effects caused by Pb and Cd combination.

Material and methods. The experiment was conducted on the outbred male rats. The animals received repeated intraperitoneal injections of water solutions of lead acetate and cadmium chloride, 3 times per week for 6 weeks. After the exposition was completed, more than 50 indices of toxic exposure (including biochemical and histo-morphological ones) were estimated in all groups of the tested animals. To assess the genotoxic effect of “in vivo” there was used amplified fragment length polymorphism (AFLP) analysis. Statistical analysis was done using Student’s t-test.

Results. We found the administration of the bioprotective formula to improve the indices of general toxicity. Genotoxicity studied using AFLP analysis of blood cells DNA was shown to be mitigated. Histo-morphological indices of Pb+Cd hepato- and nephrotoxicity improved under a bioprotective complex (BPC) administration. Blood Pb and Cd decreased during BPC administration. There was a statistically reliable decrease in the mean diameter of cardiomyocytes associated with Pb+Cd administration. These changes became less apparent with the BPC administration.

Conclusion. We developed and tested a strategy to mitigate the toxic effects of Pb and Cd at organ and organ system levels, including general toxicity, target organ toxicity (with cardiotoxicity) and genоtoxicity.

Keywords

lead, cadmium, toxicity, bioprotectors

About the authors

Larisa I. Privalova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Author for correspondence.
Email: privalovali@yahoo.com
ORCID iD: 0000-0002-1442-6737

MD, Ph.D., DSci, Head of Laboratory of Scientific Basis for Biological Prophylaxis, Department of the Toxicology and Bioprophylaxis, Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Yekaterinburg, 620014, Russian Federation.

e-mail: privalovali@yahoo.com

Russian Federation

References

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