Karyological status of buccal epithelial cells of miners with occupational lung pathologies

Cover Page

Cite item

Full Text

Abstract

Introduction. Coal dust is the main phlogogenic factor in the development of chronic dust bronchitis in miners. The functional activity of buccal epithelium largely determines the reactivity of the body and modulates the development of the pathological process. 

Material and methods. 49 miners (mean age of 51.08 ± 0.51 years) with a history of chronic dust bronchitis, 55 miners (mean age of 50.96 ± 0.37 years), without occupational diseases, and 52 men of the control group (mean age of 50.61 ± 3.27 years) were examined with the use of micro-nuclear test to determine the frequency of prevalence rate of buccal epithelial cells with micronuclei, nuclear protrusion “bubble”, “broken egg”, “tongue”, as well as binuclear cells and double-nuclei cells.

Results. In miners without occupational diseases, the frequency of the occurrence of cells with micronuclei and double-nuclei was 0.73 ± 0.99‰ and 4.25 ± 3.75‰, for the control group — 0.31 ± 0.53‰ (p = 0.0384) and 2.89±2.76‰ (p = 0.0195), respectively. Significant differences in miners with chronic dust bronchitis regarding the miners without occupational diseases were established on the following nuclear anomalies: the micronuclei (1.69 ± 1.29‰; 0.73 ± 0.99‰; p = 0.0384), protrusion “bubble” — (2.43 ± 2.08‰; 1.20 ± 1.51 ‰; p = 0.7175), the total frequency of protrusion (3.67±2.88‰; 1.81±2.01‰; p = 0.0001), binucleatedness (5.51 ± 4.28‰; 3.85 ± 4.01‰; p = 0.0129) and double-nuclei — (7.43 ± 4.45‰; 4.25 ± 3.75‰; p < 0.0001). 

Discussion. No significant associations between nuclear anomalies with work experience and presence of harmful habits (smoking) were found in any of the examined groups. 

Conclusion. Thus, factors of underground mining production induce clastic and aneugenic effects; worsening hypoxia, related with the deterioration of respiration functions, the influence of inflammatory mediators and impaired redox homeostasis significantly increase the frequency of karyological damage in buccal epithelial cells.

About the authors

Alina V. Meyer

Kemerovo State University

Author for correspondence.
Email: shapo-alina@yandex.ru
ORCID iD: 0000-0001-9952-7854

Assistant professor of the department of Genetics Kemerovo State University.

e-mail: shapo-alina@yandex.ru

Russian Federation

T. A. Tolochko

Kemerovo State University

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4645-7009
Russian Federation

A. V. Litvin

Kemerovo State University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-9910-3477
Russian Federation

V. I. Minina

Federal Research Center for coal and Coal Chemistry of the SB RAS

Email: noemail@neicon.ru
ORCID iD: 0000-0003-3485-9123
Russian Federation

Yu. E. Kulemin

Kemerovo State University; Federal Research Center for coal and Coal Chemistry of the SB RAS

Email: noemail@neicon.ru
ORCID iD: 0000-0003-1789-586X
Russian Federation

References

  1. Chen Y., Shan N., Huggins F.E., Huffman G.P. Transmission electron microscopy investigation of ultrafine coal fly ash particles. Environ. Sci. Technol. 2005; 39(4): 1144-51.
  2. Schins R.P., Borm P.J. Mechanisms and mediators in coal dust induced toxicity. Ann. Ocup. Hyg. 1999; 48(1): 7-13.
  3. Ellmerich S., Djouder N., Shjller M., Klein J.P. Production of cytokines by monocytes, ephithelial and endothelial cells activated by Streptococcus bovis. Cytokine. 2000; 12(1): 26-31.
  4. Yanagita M., Shimabucuro Y., Nozaki T., Yoshimura N., Watanabe J., Koide H. et al. IL-15 up-regulates iNOS expression and NO-production by gingival ephithelial cells. Biochem. Biophys. Res. Commun. 2002; 297(2): 329-34.
  5. Thomas P., Hollad N., Bolognesi C., Kirsch-Volders M., Bonassi S., Zeiger E. et al. Buccal micronucleus cytome assay. Nat. Protoc. 2009; 4(6): 825-37.
  6. Sycheva L.P. Full range of karyological indicators limits of variation, determining criterias and biological significance in assessing of human cytogenetic status. Meditsinskaya genetika. 2007; 6(11): 3-11. (in Russian)
  7. Volobaev V.P., Sinitsky M.Yu., Larionov A.V., Druzhinin V.G., Gafarov N.I., Minina V.I. et al. Modifying influence of occupational inflammatory diseases on the level of chromosome aberrations in coal miners. Mutagenesis. 2016; 31(2): 225-9.
  8. Holland N., Bolognesi C., Kirsch-Volders M., Bonassi S., Zeiger E., Knasmueller S. et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat. Res. 2008; 659(1-2): 93-108.
  9. Leon-Mejia G., Quintana M., Debastini R., Dias J., Espitia-Perez L., Hartmann A. et al. Genetic damage in coal miners evaluated by buccal micronucleus cytome assay. Environ. Saf. 2014; 107: 133-9.
  10. Rohr P., da Silva J., da Silva F. R., Sarmento M., Porto C., Debastiani R. et al. Evaluation of genetic damage in open-cast coal mine workers using the buccal micronucleus cytome assay. Environ. Mol. Mutagen. 2013; 54(1): 65-71.
  11. Petrashova D.A., Belisheva N.K., Pelevina I.I., Mel’nik N.A., Zol’zer F. Genotoxic effects in the buccal epithelium of the miners, occupationally exposure to natural sources of ionizing radiation. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk. 2011; 13(1): 1792-6. (in Russian)
  12. Nersesyan A. Nuclear buds in exfoliated human cells. Mutat. Res. 2005; 588(1): 64-8.
  13. Serrano-Garcia L., Montero-Montoya R. Micronuclei and chromatid buds are the result of related genotoxic events. Environ. Mol. Mutagen. 2001; 38(1): 38-45.
  14. Zeljezic D., Garaj-Vrhovac V. Chromosomal aberrations, micronuclei and nuclear buds induced in human lymphocytes by 2,4- dichlorophenoxyacetic acid pesticide formulation. Toxicology. 2004; 200(1): 39-47.
  15. Montero R., Serrano L., Davila V., Segura Y., Arrieta A., Fuentes R. et al. Metabolic polymorphisms and the micronucleus frequency in buccal epithelium of adolescents living in an urban environment. Environ. Mol. Mutagen. 2003; 42(3): 216-22.
  16. Fenech M., Crott J.W. Micronuclei, nucleoplasmic bridges and nuclear buds induced in folic acid deficient human lymphocytes-evidence for breakage–fusion–bridge cycles in the cytokinesis-block micronucleus assay. Mutat. Res. 2002; 504(1-2): 131-6.
  17. Shimizu N., Itoh N., Utiyama H., Wahl G.M. Selective entrapment of extrachromosomally amplified DNA by nuclear budding and micronucleation during S phase. J. Cell Biol. 1998; 140(6): 1307-20.
  18. Gadhia P.K., Thumbar P.P., Kevadiya B. Cytome assay of buccal epithelium for bio-monitoring genotoxic assessment of benzene exposure among petrol pump attendants. Int. J. Hum. Genet. 2010: 10(4): 239-45.
  19. Celik A., Yildirim S., Ekinci S.Y., Tasdelen B. Bio-monitoring for the genotoxic assessment in road construction works as determined by the buccal micronucleus cytome assay. Ecotoxicol. Environ. Saf. 2013; 92: 265-70.
  20. Benedetti D., Nunes E., Sarmento M.S., Porto C., Santos C.E.I., Dias J.F. et al. Genetic damage in soybean workers exposed to pesticides: Evaluation with the comet and buccal micronucleus cytome assays. Mut. Res. 2013; 752(1-2): 28-33.
  21. Kvitko K., Bandinelli E., Henriques J.A., Heuser V.D., Rohr P., da Silva F.R. et al. Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery hemicals and to coal dust during mining. Genet. Mol. Biol. 2012; 35(4): 1060-8.
  22. Meyer A.V., Tolochko T.A., Minina V.I., Timofeeva A.A. Influence of polymorphisms of DNA repair genes in the human karyological status buccal epithelial cells during exposure to radon. Ekologicheskaya genetika. 2014; XII(1): 28-38. (in Russian)
  23. Otto M., Gogvadze V., Orrenius S., Zhivotovsky B. Mitochondria, oxidative stress and cell death. Apoptosis. 2007; 12: 913-22.
  24. Yen W.L., Klionsky D.J. How to live long and prosper: autophagy, mitochondria, and aging. Physiology (Bethesda). 2008; 23: 248-62.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Meyer A.V., Tolochko T.A., Litvin A.V., Minina V.I., Kulemin Y.E.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.