Polymorphic CYPs and GSTs genes’ loci in workers exposed to chronic mercury vapor exposure

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Abstract

Introduction. The study investigated the association between polymorphic loci of xenobiotics biotransformation genes and the development of the chronic mercury intoxication (CMI) in a cohort of 120 former male workers of caustic soda production plant, who had been chronically exposed to mercury vapor. 

Material and methods. The polymorphic variants of CYP1A1 (+462Ile/Val, rs1048943), CYP1A2*F (-163C/A, rs762551), CYP2E1 (+1053C/T, rs2031920), GSTM1 and GSTT1 genes were studied. The workers were divided into two groups: group 1 included 46 subjects who had contact with mercury but without the diagnosis of CMI; group 2 consisted of 74 patients in the remote period of CMI. Two-tailed Fisher’s exact test was used to estimate group differences in allele and genotype frequencies, as well as the logistic regression analysis for 4 genetic models to detect associations of studied polymorphic loci along with the development of CMI. 

Results. The prevalence of IleVal heterozygote in group of workers without the disease (group 1, n = 46) was found to be higher (p = 0.01) if compared to patients with CMI diagnosis (group 2, n = 74), and there was revealed also the inverse association between IleVal-CYP1A1 (+ 462Ile/Val) genotype and the development of CMI (OR = 0.10, 95% CI 0.02-0.48, p <0.001). The lack of carriers of ValVal homozygotes in the examined cohort was determined. Such data show the IleVal genotype to be a marker of resistance to disease development. 

Conclusion. The obtained results indicate that the polymorphic locus CYP1A1 (+ 462 Ile/Val) could be significant in the mechanisms of CMI development which promotes the search for effective criteria for assessing individual’s susceptibility and the risk of the development of this pathology.

About the authors

Yury I. Chernyak

East-Siberian Institute of Medical and Ecological Research

Author for correspondence.
Email: yuri_chernyak@hotmail.com
ORCID iD: 0000-0001-9641-0327

MD, Ph.D., DSc, Leading researcher of the Laboratory of Immunological, biochemical, molecular and genetic research of the East-Siberian Institute of Medical and Ecological Research, Angarsk, 665827, Russian Federation. 

e-mail: yuri_chernyak@hotmail.com

Russian Federation

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