Development of a subunit vaccine against virus of african svine virus based on cd2v protein with immunoinformatics and molecular dynamics methods
- Authors: Ivanovsky A.S.1,2, Timofeev V.I.1, Kalach A.V.2, Kordonskaya Y.V.3, Marchenkova M.A.1, Pisarevsky Y.V.1, Dyakova Y.A.3, Kovalchuk M.V.1,3
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Affiliations:
- Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute
- MIREA-Russian Technology University
- National Research Centre “Kurchatov Institute”
- Issue: Vol 69, No 6 (2024)
- Pages: 987-997
- Section: КРИСТАЛЛОГРАФИЯ В БИОЛОГИИ И МЕДИЦИНЕ
- URL: https://ruspoj.com/0023-4761/article/view/673625
- DOI: https://doi.org/10.31857/S0023476124060089
- EDN: https://elibrary.ru/YHSZOA
- ID: 673625
Cite item
Abstract
A subunit vaccine against the African swine fever virus (ASF) has been designed using immunoinformatics and molecular dynamics methods. The three-dimensional structure of the selected immunogenic protein of the ASF-CD2v virus was modelled, and its topology relative to the membrane was predicted. B- and T-cell epitopes for the supramembrane part of CD2v were predicted, and their immunogenicity, allergenicity, and toxicity were evaluated. In order to facilitate the development of a subunit vaccine, the least variable site was identified on the basis of an analysis of the conservatism of the predicted epitopes. The stability of the selected supramembrane site in an aqueous salt solution was evaluated using molecular dynamics methods, and it was demonstrated that the site is structurally stable. The immunomodulation method has demonstrated that the developed candidate vaccine is capable of eliciting a sustained immune response and does not result in the development of a cytokine storm.
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About the authors
A. S. Ivanovsky
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute; MIREA-Russian Technology University
Author for correspondence.
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow; Moscow
V. I. Timofeev
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow
A. V. Kalach
MIREA-Russian Technology University
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow
Y. V. Kordonskaya
National Research Centre “Kurchatov Institute”
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow
M. A. Marchenkova
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow
Y. V. Pisarevsky
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow
Y. A. Dyakova
National Research Centre “Kurchatov Institute”
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow
M. V. Kovalchuk
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute; National Research Centre “Kurchatov Institute”
Email: a.1wanowskiy@gmail.com
Russian Federation, Moscow; Moscow
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