Mycobiota of Potato Tubers Grown in Kamchatka Krai

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Abstract

This work studies the diversity of fungi isolated from diseased potato tubers grown in Kamchatka Krai. Species identification showed that 44 of 48 studied strains belonged to the Ascomycota (classes Dothideomycetes, Sordariomycetes, Saccharomycetes, Orbiliomycetes) and 4 strains to the Basidiomycota (class Agaricomycetes). Species affiliation analysis showed that they belonged to the following species: Ascomycota – Boeremia exigua, B. foveata, Clonostachys rosea, C. solani, Fusarium avenaceum, F. merismoides, F. oxysporum, F. sporotrichioides, F. torulosum, Geotrichum candidum, Helminthosporium solani, Orbilia oligospora, Plectosphaerella sp., Trichocladium solani, Verticillium albo-atrum, Volutella sp.; Basidiomycota – Sistotrema brinkmannii, Rhizoctonia solani AG-3, Ceratobasidium sp. AG-I. The study showed that dominated pathogens were Boeremia exigua and B. foveata, Rhizoctonia solani, Helminthosporium solani, Trichocladium solani, Fusarium sp. Strains of these species were characterized by high pathogenicity. At the same time, species previously not observed on potatoes were also isolated. Among them were both nonpathogenic for potato tuber tissues (Sistotrema brinkmannii, Ceratobasidium sp. AG-I, F. merismoides) and weakly pathogenic Volutella sp. Strains of Arthrobotrys oligospora were found on tubers affected by the nematode. For a more complete understanding of the potato-associated mycobiota, it is necessary to continue research in unexplored areas of the Kamchatka Territory and the Russian Far East as a whole.

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About the authors

D. N. Skokov

Peoples’ Friendship University of Russia

Author for correspondence.
Email: qases-0987@yandex.ru
Russian Federation, 117198 Moscow

A. A. Tsindeliania

Peoples’ Friendship University of Russia

Email: archil7876@yandex.ru
Russian Federation, 117198 Moscow

O. I. Khasbiullina

Kamchatka Research Institute of Agriculture, branch of the FRC N. I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: khasbiullina@kamniish.ru
Russian Federation, Kamchatka krai, 684033

V. V. Gainatulina

Kamchatka Research Institute of Agriculture, branch of the FRC N. I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: vera30.10@mail.ru
Russian Federation, Kamchatka krai, 684033

S. N. Elansky

Peoples’ Friendship University of Russia; Lomonosov Moscow State University

Email: snelansky@mail.ru
Russian Federation, 117198, Moscow; 119991, Moscow

E. M. Chudinova

Peoples’ Friendship University of Russia

Email: chudiel@mail.ru
Russian Federation, 117198 Moscow

References

  1. A’Hara D. Detection and identification of Phoma pathogens of potato. Meth. Mol. Biol. 2015. V. 1302. P. 17–27. https://doi.org/10.1007/978-1-4939-2620-6_2
  2. Alam M.W., Malik A., Rehman A. et al. First report of potato wilt caused by Plectosphaerella cucumerina in Pakistan. J. Plant. Pathol. 2021. V. 103. P. 687. https://doi.org/10.1007/s42161-021-00771-y
  3. Belosokhov A., Yarmeeva M., Kokaeva L. et al. Trichocladium solani sp. nov. – a new pathogen on potato tubers causing yellow rot. J. Fungi. 2022. V. 8. e1160. https://doi.org/10.3390/jof8111160
  4. Belosokhov A.F., Yarmeeva M.M., Mislavsky S.M. et al. Mycobiota of potato tubers. Mikologiya i fitopatologiya. 2023. Vol. 57(2). P. 123–133. (In Russ.). https://doi.org/10.31857/S0026364823020046
  5. Chudinova E.M., Kokaeva L. Yu., Elansky S.N. et al. The occurrence of thiabendazole-resistant isolates of Helminthosporium solani on potato seed tubers in Russia. J. Plant Dis. Protect. 2020. V. 127. P. 421–423. http://dx.doi.org/10.1007/s41348-020-00313-1
  6. Díaz-Nájera J.F., Ayvar-Serna S., Vargas-Hernández M. et al. First report of Ceratobasidium sp. causing root rot of Crotalaria juncea in Mexico. New Dis. Rep. 2023. V. 47. e12165. https://doi.org/10.1002/ndr2.12165
  7. Elansky A.S., Mislavskiy S.M., Chudinova E.M. et al. Fusarium species affecting potato tubers and tomato fruits in Uganda. Mikologiya i fitopatologiya. 2024. V. 58 (2). P. 163–174. http://dx.doi.org/10.31857/S0026364824020077
  8. Elansky S.N., Chudinova E.M., Elansky A.S. et al. Microorganisms in spent water-miscible metalworking fluids as a resource of strains for their disposal. J. Cleaner Production. 2022. V. 350. e131438. https://doi.org/10.1016/j.jclepro.2022.131438
  9. FAO. 2022. https://www.fao.org/faostat/en/#data/FBS. Accessed 15.11.2024.
  10. Gao J., Zhang Y.Y., Zhao X.J. et al. First report of Potato wilt caused by Plectosphaerella cucumerina in Inner Mongolia, China. Plant Dis. 2016. V. 100 (12). P. 2523. https://doi.org/10.1094/PDIS-01-16-0028-PDN
  11. Gavrilova O., Orina A., Trubin I. et al. Identification and pathogenicity of fusarium fungi associated with dry rot of potato tubers. Microorganisms. 2024. V. 12 (3). P. 598. http://dx.doi.org/10.3390/microorganisms12030598.
  12. Innis M.A., Gelfand D.H., Sninsky J.J. et al. PCR Protocols: A guide to methods and applications. Academic Press, San Diego, 1990. P. 315.
  13. Kokaeva L.Y., Yarmeeva M.M., Kokaeva Z.G. et al. Phylogenetic study of Alternaria potato and tomato pathogens in Russia. Diversity. 2022. V. 14 (8). P. 685. https://doi.org/10.3390/d14080685
  14. Kopina M.B., Uvarova D.A., Shukhin D.I. et al. On some pycnidial fungi on vegetative potatoes in the Non-Black Earth Region of central Russia. Fitosanitariya. Karantin rasteniy. 2021. N3. P. 27–39. (In Russ.). https://doi.org/10.69536/FKR.2021.49.59.001
  15. Kutuzova I.A., Kokaeva L.Y., Pobendinskaya M.A. et al. Resistance of Helminthosporium solani strains to selected fungicides applied for tuber treatment. J. Plant Pathol. 2017. V. 99 (3). P. 635–642. http://dx.doi.org/10.4454/jpp.v99i3.3950
  16. Loit K., Soonvald L., Astover A. et al. Temporal and cultivar-specific effects on potato root and soil. Fungal Diversity. 2020. V.10 (10). e1535. https://doi.org/10.3390/agronomy10101535
  17. Mardanova A., Lutfullin M., Hadieva G. et al. Structure and variation of root-associated microbiomes of potato grown in alfisol. World J. Microbiol. Biotechnol. 2019. V. 35 (12). P. 181. https://link.springer.com/article/10.1007/s11274-019-2761-3
  18. Meng P.P., Liu X., Qiu H.Z. et al. Fungal population structure and its biological effect in rhizosphere soil of continuously cropped potato. Ying Yong Sheng tai xue bao – The Journal of Applied Ecology. 2012. V. 23 (11). P. 3079–3086.
  19. O’Donnell K., Kistler H.C., Cigelnik E. et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. PNAS. 1998. V. 95 (5). P. 2044–2049. https://doi.org/10.1073/pnas.95.5.2044
  20. Pilipova Yu., Shaldyaeva E. Monitoring of pests as a basis for phytosanitary optimization of potato agroecosystems. Innovatsii i prodovolstvennaya bezopasnost. 2019. N1. P. 42–50. (In Russ.).
  21. Potatosystem. 2024. https://potatosystem.ru/kartofelevodstvo-dalnevostochnyj-federalnyj-okrug. Accessed 15.11.2024.
  22. Qin S., Yeboah S., Xu X. et al. Analysis on fungal diversity in rhizosphere soil of continuous Cropping potato subjected to different furrow-ridge mulching managements. Front. Microbiol. 2017. V. 10 (8). Art. 845. https://doi.org/10.3389/fmicb.2017.00845
  23. Xie T., Shen S., Hao Y. et al. Comparative analysis of microbial community diversity and dynamics on diseased tubers during potato storage in different regions of Qinghai China. Front. Genet. 2022. V. 13. e818940. https://doi.org/10.3389/fgene.2022.818940
  24. Yarmeeva M., Kutuzova I., Kurchaev M. et al. Colletotrichum species on cultivated Solanaceae crops in Russia. Agriculture 2023. V. 13. P. 511. https://doi.org/10.3390/agriculture13030511
  25. Yarmeeva M.M., Kokaeva L.Y., Chudinova E.M. et al. Anastomosis groups and sensitivity to fungicides of Rhizoctonia solani strains isolated from potato in Russia. J. Plant Dis. Prot. 2021. V. 128. P. 1253–1261. http://dx.doi.org/10.1007/s41348-021-00490-7
  26. Zhang X., Zhang H., Jiang Z. et al. A new strain of Volutella citrinella with nematode predation and nematicidal activity, isolated from the cysts of potato cyst nematodes in China. BMC Microbiol. 2021. V. 21. P. 323. https://doi.org/10.1186/s12866-021-02385-x
  27. Zheng J., Yao L., Liu C. et al. First report of Ceratobasidium sp. AG-I (binucleate Rhizoctonia) causing root rot of Hedychium coronarium Koen in China. Can. J. Plant Pathol. 2023. V. 45 (2). P. 168–175. https://doi.org/10.1080/07060661.2022.2162607
  28. Белосохов А.Ф., Ярмеева М.М., Миславский С.М. и др. (Belosokhov et al.) Микобиота клубней картофеля. // Микология и фитопатология. 2023. Т. 57. № 2. С. 123–133.
  29. Копина М.Б., Уварова Д.А., Шухин Д.И. и др. (Kopina et al.) О некоторых пикнидиальных грибах на вегетирующем картофеле в Нечерноземье средней полосы России // Фитосанитария. Карантин растений. 2021. № 3. С. 27–39.
  30. Пилипова Ю., Шалдяева Е. (Pilipova, Shaldyaeva) Мониторинг вредных организмов как основа фитосанитарной оптимизации агроэкосистем картофеля // Инновации и продовольственная безопасность. 2019. № 1. С. 42–50.

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2. Fig. 1. Pathogenicity of strains in potato slice test. The diameter of the lesion on the seventh day of incubation at 25°C is shown in red, the seventh day of incubation at 10°C is shown in grey, and the colony growth during the second week of incubation is shown in blue. Only strains that showed pathogenic properties are shown in the figure.

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3. Fig. 2. Phylogram of fungi of the genus Fusarium, constructed based on the analysis of a part of the tef 1α gene. Constructed in the MegaX program using the ML maximum likelihood algorithm.

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