Unsteady-State Mass Transfer in Gels with Microorganisms

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Abstract

Hydrogels are promising for use in tissue engineering as matrices for cell incubation and with subsequent use of such biomaterial systems as bioinks suitable for 3D bioprinting. The heat and mass transfer in hydrogel materials based on agarose and gelatin with model microorganisms incubated in the bulk of the gel are studied by the optical methods of spectroscopy and microscopy. The propagation of the fronts of the nutrient medium in pure gelatin hydrogels and mixed hydrogels based on agarose with the addition of gelatin are compared to determine the diffusion properties of the mixed hydrogels that can ensure the delivery of nutrient components to microorganisms. New data are obtained on the degree of heterogeneity in the growth of microorganisms during their bulk incubation under various temperature conditions of incubation, which is necessary to control the properties of bioink in bioprinting. An analytical description of the curve of cell growth in the gel is proposed, and on its basis an expression for determining the cell growth rate is obtained. A numerical model is developed to describe the absorption of nutrients by cells during their growth.

About the authors

D. P. Khramtsov

RTU MIREA—Russian Technological University; RTU MIREA—Russian Technological University

Email: dp@khramtsov.net
107023, Moscow, Russia; 119571, Moscow, Russia

O. A. Khramtsov

Moscow Polytechnic University

Email: dp@khramtsov.net
107023, Moscow, Russia

B. G. Pokusaev

Moscow Polytechnic University; RTU MIREA—Russian Technological University

Email: dp@khramtsov.net
107023, Moscow, Russia; 119571, Moscow, Russia

A. V. Vyazmin

Moscow Polytechnic University; RTU MIREA—Russian Technological University

Email: dp@khramtsov.net
107023, Moscow, Russia; 119571, Moscow, Russia

D. A. Nekrasov

Moscow Polytechnic University; RTU MIREA—Russian Technological University

Author for correspondence.
Email: dp@khramtsov.net
107023, Moscow, Russia; 119571, Moscow, Russia

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Copyright (c) 2023 Д.П. Храмцов, О.А. Сулягина, Б.Г. Покусаев, А.В. Вязьмин, Д.А. Некрасов