Separation and concentration of biologically active compounds by capillary electrophoresis using modifiers based on imidazolium cation
- Authors: Ganieva A.S.1, Kartsova L.A.1
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Affiliations:
- St. Petersburg State University
- Issue: Vol 80, No 4 (2025)
- Pages: 427-436
- Section: ORIGINAL ARTICLES
- Submitted: 05.06.2025
- URL: https://ruspoj.com/0044-4502/article/view/683049
- DOI: https://doi.org/10.31857/S0044450225040073
- EDN: https://elibrary.ru/aksfdl
- ID: 683049
Cite item
Abstract
A cationic covalent coating of quartz capillary walls based on imidazolium cation was synthesised; the reproducibility by electroosmotic flow (marker dimethylformamide) was 99 %. The conditions (concentration and pH of background electrolyte, nature and volume of organic additive) for their separation by capillary electrophoresis were optimised on model systems of phenyl- and indolecarboxylic acids (phenyl lactic, phenylanthanoic, phenyl butanoic, indole lactic, indolpropionic, indolacrylic hydroxymindal, homogentisic, homovanilinic, hydroxindoleacetic acids): 10 mM phosphate buffer solution with pH 4.2 with addition of 10 vol. % acetonitrile. The resolution factors of neighbouring pairs of analytes ranged from 1.7 to 18.9. The possibility of intracapillary concentration of phenyl- and indolecarboxylic acids using the synthesised coating was shown. Electrostacking allowed to concentrate the analytes 106–512 times and to lower the detection limits to 4–72 ng/ml.
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About the authors
A. Sh. Ganieva
St. Petersburg State University
Author for correspondence.
Email: st110520@student.spbu.ru
Russian Federation, Saint-Petersburg
L. A. Kartsova
St. Petersburg State University
Email: st110520@student.spbu.ru
Russian Federation, Saint-Petersburg
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