Gas chromatographic determination of ethanol in soft drinks using vapor-phase drop microextraction

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Abstract

A simple express procedure for gas chromatographic determination of ethanol in soft drinks is proposed (kvass, grape juice, non-alcoholic beer), based on headspace microextraction of the analyte into a drop (1 μL) of distilled water located above the surface of the analyzed liquid on the tip of the needle of a standard microsyringe for gas chromatography. The detection limit of ethanol is (3–6) × 10–5 vol. %, the parallel determination time, including headspace extraction, does not exceed 5 min. Propanol-2, which is not detected in beverages, is used as an internal standard. The correctness of the procedure is confirmed by the added–found method, the relative standard deviation does not exceed 7 % at an ethanol concentration in the sample of 10–2 vol. %.

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About the authors

O. V. Rodinkov

Saint Petersburg State University

Author for correspondence.
Email: o.rodinkov@spbu.ru

Institute of Chemistry

Russian Federation, Universitetskii pr. 26, Petrodvorets, Saint Petersburg

K. N. Davletbaeva

Saint Petersburg State University

Email: o.rodinkov@spbu.ru

Institute of Chemistry

Russian Federation, Universitetskii pr. 26, Petrodvorets, Saint Petersburg

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Supplementary files

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2. Fig. 1. Schematic diagram of the setup for vapor-phase droplet microextraction. 1 – analyzed solution; 2 – drop of extractant; 3 – polymer septum with aluminum cap; 4 – microsyringe; 5 – foot with stand.

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3. Fig. 2. Dynamics of changes in the concentration of ethanol cэ in a drop of aqueous extractant depending on the time of its stay above the model aqueous solution with an ethanol concentration of 0.1 vol.%. 1 – with stirring; 2 – without stirring.

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4. Fig. 3. Dependence of the analytical signal (ratio of the peak areas of ethanol and the internal standard) on the concentration of ethanol in model aqueous solutions.

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5. Fig. 4. Chromatogram obtained during the analysis of kvass using an internal standard (propanol-2) with a concentration of 0.1 vol.%. 1 – ethanol; 2 – propanol-2.

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