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IMMUNOPHENOTYPE OF MESENCHYMAL STEM CELLS DERIVED FROM EPICARDIAL AND PERIVASCULAR ADIPOSE TISSUE IN PATIENTS WITH CARDIOVASCULAR DISEASES
- Authors: Uchasova E.G.1, Dyleva Y.A.1, Belik E.V.1, Matveeva V.G.1, Gusev S.M.1, Tarasova O.L.2, Gruzdeva O.V.1,2
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Affiliations:
- Research Institute for Complex Issues of Cardiovascular Diseases
- Kemerovo State Medical University Ministry of Health of the Russian Federation
- Issue: Vol 59, No 3 (2023)
- Pages: 215-222
- Section: EXPERIMENTAL ARTICLES
- URL: https://ruspoj.com/0044-4529/article/view/648057
- DOI: https://doi.org/10.31857/S0044452923030099
- EDN: https://elibrary.ru/YHWKHY
- ID: 648057
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Abstract
Aim: to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in patients with coronary heart disease and acquired heart defects. Results: In cell culture obtained from epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT) (2nd passage) in patients with both coronary heart disease and acquired heart defects, a high (over 90%) expression of membrane proteins characteristic of stem cells. In addition to the main population, with both in the EAT culture and in the PVAT, there were 2 minor ones: 1 – CD90-, CD105+, CD34-/+, CD73+, CD45- is presumably endothelial population, 2 – CD90+, CD105-, CD34-, CD73-, CD45- is the smallest population.
Conclusion: In the early stages of cultivation, cells of the stromal vascular fraction isolated from epicardial and perivascular adipose tissue express surface markers characteristic of adipose tissue stem cells.
About the authors
E. G. Uchasova
Research Institute for Complex Issues of Cardiovascular Diseases
Author for correspondence.
Email: evg.uchasova@yandex.ru
Russia, Kemerovo
Yu. A. Dyleva
Research Institute for Complex Issues of Cardiovascular Diseases
Email: evg.uchasova@yandex.ru
Russia, Kemerovo
E. V. Belik
Research Institute for Complex Issues of Cardiovascular Diseases
Email: evg.uchasova@yandex.ru
Russia, Kemerovo
V. G. Matveeva
Research Institute for Complex Issues of Cardiovascular Diseases
Email: evg.uchasova@yandex.ru
Russia, Kemerovo
S. M. Gusev
Research Institute for Complex Issues of Cardiovascular Diseases
Email: evg.uchasova@yandex.ru
Russia, Kemerovo
O. L. Tarasova
Kemerovo State Medical University Ministry of Health of the Russian Federation
Email: evg.uchasova@yandex.ru
Russia, Kemerovo
O. V. Gruzdeva
Research Institute for Complex Issues of Cardiovascular Diseases; Kemerovo State Medical University Ministry of Health of the Russian Federation
Email: evg.uchasova@yandex.ru
Russia, Kemerovo; Russia, Kemerovo
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