IMMUNOPHENOTYPE OF MESENCHYMAL STEM CELLS DERIVED FROM EPICARDIAL AND PERIVASCULAR ADIPOSE TISSUE IN PATIENTS WITH CARDIOVASCULAR DISEASES

E. G. Uchasova; Учасова Е. Г.; Yu. A. Dyleva; Дылева Ю. А.; E. V. Belik; Белик Е. В.; V. G. Matveeva; Матвеева В. Г.; S. M. Gusev; Гусев С. М.; O. L. Tarasova; Тарасова О. Л.; O. V. Gruzdeva; Груздева О. В.

doi:10.31857/S0044452923030099

IMMUNOPHENOTYPE OF MESENCHYMAL STEM CELLS DERIVED FROM EPICARDIAL AND PERIVASCULAR ADIPOSE TISSUE IN PATIENTS WITH CARDIOVASCULAR DISEASES

Authors: Uchasova E.G.¹, Dyleva Y.A.¹, Belik E.V.¹, Matveeva V.G.¹, Gusev S.M.¹, Tarasova O.L.², Gruzdeva O.V.¹^,2
Affiliations:
1. Research Institute for Complex Issues of Cardiovascular Diseases
2. Kemerovo State Medical University Ministry of Health of the Russian Federation
Issue: Vol 59, No 3 (2023)
Pages: 215-222
Section: EXPERIMENTAL ARTICLES
URL: https://ruspoj.com/0044-4529/article/view/648057
DOI: https://doi.org/10.31857/S0044452923030099
EDN: https://elibrary.ru/YHWKHY
ID: 648057

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Abstract

Aim: to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in patients with coronary heart disease and acquired heart defects. Results: In cell culture obtained from epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT) (2nd passage) in patients with both coronary heart disease and acquired heart defects, a high (over 90%) expression of membrane proteins characteristic of stem cells. In addition to the main population, with both in the EAT culture and in the PVAT, there were 2 minor ones: 1 – CD90-, CD105+, CD34-/+, CD73+, CD45- is presumably endothelial population, 2 – CD90+, CD105-, CD34-, CD73-, CD45- is the smallest population.
Conclusion: In the early stages of cultivation, cells of the stromal vascular fraction isolated from epicardial and perivascular adipose tissue express surface markers characteristic of adipose tissue stem cells.

Keywords

mesenchymal stem cells, adipose tissue, ischemic heart disease, acquired heart disease

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