Cryoprotectant based on a glass-forming aqueous solution of magnesium acetate

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Abstract

For the first time a cryoprotectant based on glass-forming aqueous solution of magnesium acetate, a metal vital for the human body, was obtained and studied by DSC method. This cryoprotectant – Mg(CH3COO)2 ∙ 12H2O – surpasses the available analogues by the following parameters: it has a high glass-forming ability (it passes from the glassy state to the liquid state without crystallization), is non-toxic and easy to obtain. Its cryoprotective ability, proved on chicken egg white, does not depend on the rate of cooling and heating. It is shown that among glass-forming solutions of Mg(CH3COO)2–H2O system there are five more potential cryoprotectants and preservative for hypothermic storage of biological material. The molecular mechanism preventing damage and death of biological material placed in solutions of the Mg(CH3COO)2–H2O system favourable for cryopreservation has been established using the density functional theory method.

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About the authors

I. A. Kirilenko

Kurnakov Institute of General and Inorganic Chemistry Russian Academy of Sciences

Author for correspondence.
Email: iakirilenko@mail.ru
Russian Federation, Leninskii prosp. 31, Moscow, 119991

E. G. Tarakanova

Kurnakov Institute of General and Inorganic Chemistry Russian Academy of Sciences

Email: iakirilenko@mail.ru
Russian Federation, Leninskii prosp. 31, Moscow, 119991

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2. Fig. 1. DSC thermogram of a sample of Mg(CH3COO)2 ∙ 17H2O composition that is unstable to crystallization.

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3. Fig. 2. DSC thermograms: 1 – chicken egg white, 2 – matrix Mg(CH3COO)2∙12H2O, 3 – matrix +10 wt. % chicken egg white, 4 – matrix +30 wt. % chicken egg white.

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4. Fig. 3. Structure of complexes Mg2+ ∙ 6H2O (a) and Mg2+ ∙ 18H2O (b).

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5. Fig. 4. Dependence of the energy of the hydrogen bond between the n-th and (n-1)-th hydration shells of the Mg2+ ion on n (1). The value of the energy of the hydrogen bond in water (2).

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6. Fig. 5. Structure of fragments of polymer chains formed in aqueous solutions of magnesium acetate of compositions 1:6 (a) and 1:11 (b).

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