Interval inhalation application of an oxygen-helium mixture reverses the effects of cerebral arterial air embolism

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Abstract

Oxygen-helium mixture demonstrates marked efficacy of helium mixtures in animal models of ischemia/reperfusion, which opens the possibility of its use as an emergency measure for therapy of vascular embolism. We modelled cerebral arterial air embolism by injecting an air bubble into the internal carotid artery of awake rats. Inhalation of a heated oxygen-helium mixture immediately after modelling arterial embolism normalizes physiological abnormalities and prevents ischemic brain damage, whereas application of a heated oxygen-helium mixture 2 hours after modelling arterial embolism significantly worsens the condition of the animals.

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About the authors

V. A. Palikov

Institute of Biomedical Problems of the Russian Academy of Sciences; Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Moscow; Pushchino

N. B. Pavlov

Institute of Biomedical Problems of the Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Moscow

A. M. Ismailova

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Pushchino

R. R. Amirov

Institute of Biomedical Problems of the Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Moscow

I. A. Dyachenko

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Pushchino

N. A. Borozdina

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Pushchino

A. V. Bervinova

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Pushchino

A. T. Logunov

Closed joint stock company «Specialized Design Bureau of Experimental Equipment at the Institute oof Medical and Biological Problems of the Russian Academy of Sciences»

Email: baranov-vm@mail.ru
Russian Federation, Khimki

A. N. Murashev

Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: baranov-vm@mail.ru
Russian Federation, Pushchino

V. M. Baranov

Institute of Biomedical Problems of the Russian Academy of Sciences

Author for correspondence.
Email: baranov-vm@mail.ru
Russian Federation, Moscow

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2. Fig. 1. Results of functional tests before CAE modeling and 24 hours after CAE modeling. (a) – body temperature, (б) – minute respiratory volume, (в) – blood pressure, (г) – horizontal activity in the open field. Note: * – p < 0.05 in comparison with CAE group, + – p < 0.05 in comparison with parameters before CAE modelling of one group.

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3. Fig. 2. Histological images of frontal brain slices, 24 hours after CAE. Hematoxylin and eosin staining. Magnification x100. (a) – ischemic stroke foci in an animal with CAE; (б) – brain of an animal with CAE and HOM therapy immediately after CAE; (в) – brain of an animal with CAE and HOM therapy one hour after CAE; (г) – hemorrhagic stroke foci in an animal with CAE and HOM therapy 2 hours after CAE.

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