Synthesis of Liposomes Conjugated with CpG Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

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Abstract

The synthesis of lipid conjugate of immunostimulatory oligodeoxyribonucleotide CpG-ODN (PD-CpG-DOPE) is described. Liposomes loaded with a composition of T-cell epitopes of the SARS-CoV-2 virus (7 peptides) and carrying PD-CpG-DOPE conjugate in the membrane, including lyophilized liposomes suitable for long-term storage, were prepared. In vitro experiments on mouse peritoneal exudate cells showed a tendency to increase the immunogenicity of liposomes with peptides when PD-CpG-DOPE conjugate was introduced into the lipid bilayer, compared with the addition of the (commercial) phosphorothioate derivative of CpG-ODN in solution.

About the authors

D. S. Tretiakova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: elvod@lipids.ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

T. L. Azhikina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: elvod@lipids.ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

I. A. Boldyrev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: elvod@lipids.ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

E. V. Svirshchevskaya

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: elvod@lipids.ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

E. L. Vodovozova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: elvod@lipids.ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

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Copyright (c) 2023 Д.С. Третьякова, Т.Л. Ажикина, И.А. Болдырев, Е.В. Свирщевская, Е.Л. Водовозова