Synthesis of Liposomes Conjugated with CpG Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

D. S. Tretiakova; Третьякова Д. С.; T. L. Azhikina; Ажикина Т. Л.; I. A. Boldyrev; Болдырев И. А.; E. V. Svirshchevskaya; Свирщевская Е. В.; E. L. Vodovozova; Водовозова Е. Л.

doi:10.31857/S0132342323040437

Synthesis of Liposomes Conjugated with CpG Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

Authors: Tretiakova D.S.¹, Azhikina T.L.¹, Boldyrev I.A.¹, Svirshchevskaya E.V.¹, Vodovozova E.L.¹
Affiliations:
1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Issue: Vol 49, No 4 (2023)
Pages: 434-440
Section: ПИСЬМА РЕДАКТОРУ
URL: https://ruspoj.com/0132-3423/article/view/670594
DOI: https://doi.org/10.31857/S0132342323040437
EDN: https://elibrary.ru/OEDGQI
ID: 670594

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Abstract

The synthesis of lipid conjugate of immunostimulatory oligodeoxyribonucleotide CpG-ODN (PD-CpG-DOPE) is described. Liposomes loaded with a composition of T-cell epitopes of the SARS-CoV-2 virus (7 peptides) and carrying PD-CpG-DOPE conjugate in the membrane, including lyophilized liposomes suitable for long-term storage, were prepared. In vitro experiments on mouse peritoneal exudate cells showed a tendency to increase the immunogenicity of liposomes with peptides when PD-CpG-DOPE conjugate was introduced into the lipid bilayer, compared with the addition of the (commercial) phosphorothioate derivative of CpG-ODN in solution.

Keywords

Т-cells, epitopes, SARS-CoV-2, vaccines, peptides, liposomes, CpG-ODN

References

Apostolopoulos V., Bojarska J., Feehan J., Matsoukas J., Wolf W. // Front. Pharmacol. 2022. V. 13. P. 914467. https://doi.org/10.3389/fphar.2022.914467
Di Natale C., La Manna S., De Benedictis I., Brandi P., Marasco D. // Front Pharmacol. 2020. V. 11. P. 578382. https://doi.org/10.3389/fphar.2020.578382
Третьякова Д.С., Водовозова Е.Л. // Биол. мембраны. 2022. Т. 39. С. 85–106. [Tretiakova D.S., Vodovozova E.L. // Biochem. (Mosc.) Suppl. Ser. A Membr. Cell Biol. 2022. V. 16. P. 1–20.] https://doi.org/10.1134/s1990747822020076
Nisini R., Poerio N., Mariotti S., De Santis F., Fraziano M. // Front. Immunol. 2018. V. 9. P. 155. https://doi.org/10.3389/fimmu.2018.00155
Lee Y., Lee Y.S., Cho S.Y., Kwon H.J. // Adv. Protein Chem. Struct. Biol. 2015. V. 99. P. 75–97. https://doi.org/10.1016/bs.apcsb.2015.03.004
Heuts J., Varypataki E.M., van der Maaden K., Romeijn S., Drijfhout J.W., van Scheltinga A.T., Ossendorp F., Jiskoot W. // Pharm. Res. 2018. V. 35. P. 207. https://doi.org/10.1007/s11095-018-2490-6
Dhakal S., Cheng X., Salcido J., Renu S., Bondra K., Lakshmanappa Y.S., Misch C., Ghimire S., Feliciano-Ruiz N., Hogshead B., Krakowka S., Carson K., McDonough J., Lee C.W., Renukaradhya G.J. // Int. J. Nanomedicine. 2018. V. 13. P. 6699–6715. https://doi.org/10.2147/ijn.s178809
Белявцев А.Н., Шастина Н.С., Куприянов В.В., Николаева Л.И., Мельникова М.В., Колесанова Е.Ф., Шимчишина М.Ю., Капустин И.В. // Биоорг. химия. 2022. Т. 48. С. 453–460. [Belyavtsev A.N., Shastina N.S., Kupriyanov V.V., Nikolaeva L.I., Melnikova M.V., Kolesanova E.F., Shimchishina M.Yu., Kapustin I.V. // Russ. J. Bioorg. Chem. 2022. V. 48. P. 621–627.] https://doi.org/10.1134/S1068162022030049
Gayed P.M. // Yale J. Biol. Med. 2011. V. 84. P. 131–138.
Hemmi H., Takeuchi O., Kawai T., Kaisho T., Sato S., Sanjo H., Matsumoto M., Hoshino K., Wagner H., Takeda K., Akira S. // Nature. 2000. V. 408. P. 740–745. https://doi.org/10.1038/35047123
Hanagata N. // Int. J. Nanomedicine. 2012. V. 7. P. 2181–2195. https://doi.org/10.2147/ijn.s30197
Nikoofal-Sahlabadi S., Riahi M.M., Sadri K., Badiee A., Nikpoor A.R., Jaafari M.R. // Eur. J. Pharm. Sci. 2018. V. 119. P. 159–170. https://doi.org/10.1016/j.ejps.2018.04.018
Lahoud M.H., Ahmet F., Zhang J.G., Meuter S., Policheni A.N., Kitsoulis S., Lee C.N., O’Keeffe M., Sullivan L.C., Brooks A.G., Berry R., Rossjohn J., Mintern J.D., Vega-Ramos J., Villadangos J.A., Nicola N.A., Nussenzweig M.C., Stacey K.J., Shortman K., Heath W.R., Caminschi I. // Proc. Natl. Acad. Sci. USA. 2012. V. 109. P. 16270–16275. https://doi.org/10.1073/pnas.1208796109
Ignacio B.J., Albin T.J., Esser-Kahn A.P., Verdoes M. // Bioconjug. Chem. 2018. V. 29. P. 587−603. https://doi.org/10.1021/acs.bioconjchem.7b00808
Levenson E.A., Kiick K.L. // Acta Biomater. 2014. V. 10. P. 1134–1145. https://doi.org/10.1016/j.actbio.2013.11.022
Clauson R.M., Berg B., Chertok B. // Bioconjug. Chem. 2019. V. 30. P. 561–567. https://doi.org/10.1021/acs.bioconjchem.9b00091
Chatzikleanthous D., Schmidt S.T., Buffi G., Paciello I., Cunliffe R., Carboni F., Romano M.R., O’Hagan D.T., D’Oro U., Woods S., Roberts C.W., Perrie Y., Adamo R. // J. Control. Release. 2020. V. 323. P. 125–137. https://doi.org/10.1016/j.jconrel.2020.04.001
Andrews C.D., Provoda C.J., Ott G., Lee K.D. // Bioconjug. Chem. 2011. V. 22. P. 1279–1286. https://doi.org/10.1021/bc100436y
Lai C., Duan S., Ye F., Hou X., Li X., Zhao J., Yu X., Hu Z., Tang Z., Mo F., Yang X., Lu X. // Theranostics. 2018. V. 8. P. 1723–1739. https://doi.org/10.7150/thno.22056
Третьякова Д.С., Алексеева А.С., Онищенко Н.Р., Болдырев И.А., Егорова Н.С., Васина Д.В., Гущин В.А., Чернов А.С., Телегин Г.Б., Казаков В.А., Плохих К.С., Коновалова М.В., Свирщевская Е.В., Водовозова Е.Л. // Биоорг. химия. 2023. Т. 49. С. 48–64. [Tretiakova D.S., Alekseeva A.S., Onishchenko N.R., Boldyrev I.A., Egorova N.S., Vasina D.V., Gushchin V.A., Chernov A.S., Telegin G.B., Kazakov V.A., Plokhikh K.S., Konovalova M.V., Svirshchevskaya E.V., Vodovozova E.L. // Russ. J. Bioorg. Chem. 2022. V. 48. Suppl. 1. P. S23–S37.] https://doi.org/10.1134/S1068162022060255
Meng W., Yamazaki T., Nishida Y., Hanagata N. // BMC Biotechnol. 2011. V. 11. P. 88. https://doi.org/10.1186/1472-6750-11-88
Mouritsen O.G., Jørgensen K. // Chem. Phys. Lipids. 1994. V. 73. P. 3–25. https://doi.org/10.1016/0009-3084(94)90171-6
Mansourian M., Badiee A., Jalali S.A., Shariat S., Yazdani M., Amin M., Jaafari M.R. // Immunol. Lett. 2014. V. 162. P. 87–93. https://doi.org/10.1016/j.imlet.2014.07.008
Schmidt S.T., Foged C., Korsholm K.S., Rades T., Christensen D. // Pharmaceutics. 2016. V. 8. P. 7. https://doi.org/10.3390/pharmaceutics8010007
Engler O.B., Schwendener R.A., Dai W.J., Wolk B., Pichler W., Moradpour D., Brunner T., Cerny A. // Vaccine. 2004. V. 23. P. 58–68. https://doi.org/10.1016/j.vaccine.2004.05.009
Kryukova E.V., Egorova N.S., Kudryavtsev D.S., Lebedev D.S., Spirova E.N., Zhmak M.N., Garifulina A.I., Kasheverov I.E., Utkin Y.N., Tsetlin V.I. // Front. Pharmacol. 2019. V. 10. P. 748. https://doi.org/10.3389/fphar.2019.00748
Magano J., Conway B.G., Farrand D., Lovdahl M., Maloney M.T., Pozzo M.J., Teixeira J.J., Rizzo J., Tumelty D. // Synthesis. 2014. V. 46. P. 1399–1406. https://doi.org/10.1055/s-0033-1340980
Germann M.W. // Nucleic Acids NMR Spectroscopy. Departments of Chemistry and Biology, Georgia State University, 2014. http://tesla.ccrc.uga.edu/courses/BioNMR2014/lectures/pdfs/NMR_14_mwgL1.pdf