Electrostatic potentials during adsorption and photochemical reactions of pyranine on bilayer lipid membranes

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Аннотация

Adsorption and photochemical reactions of pyranine on a bilayer lipid membrane (BLM) have been studied by measuring electrostatic potentials at the membrane–water interface. The dependence of the electrostatic potentials due to the adsorption of pyranine on its concentration in solution is described by the Gouy–Chapman theory assuming that anions with three charged groups are adsorbed on the membrane. No significant changes in the boundary potential were found when BLM with pyranine adsorbed on it was illuminated. Significant changes in the potential were observed if molecules of styryl dyes di-4-ANEPPS or RH-421 were adsorbed on BLM in addition to pyranine. The sign and magnitude of these changes correspond to the disappearance of the dipole potential created by styryl dye molecules on the BLM. The rate of potential disappearance was proportional to pyranine concentration and illumination intensity. The disappearance of the potential can be caused either by the binding of protons released from the pyranine molecule to the dye molecules with their subsequent desorption from the BLM or by their destruction. Pyranine and styryl dye molecules can form complexes at the BLM boundary. This is evidenced by experiments in which the sum of the potential changes caused by their adsorption separately differed significantly from the change in the boundary potential during their simultaneous adsorption. The kinetics of the disappearance of the dipole potential of BLM with styryl dyes upon excitation of pyranine turned out to be similar to that observed earlier with another compound, 2-methoxy-5-nitrophenyl sodium sulfate, which releases protons at the membrane boundary upon illumination (Konstantinova et al., 2021. Biochem. (Mosc.), Suppl. Series A: Membr. Cell Biol. 15 (2), 142–146). This suggests that it is associated with the desorption of dye molecules from the membrane, due to the binding of protons released from excited pyranin molecules to them.

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Авторлар туралы

V. Sokolov

Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: sokolov.valerij@gmail.com
Ресей, Moscow, 119071

V. Tashkin

Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences

Email: sokolov.valerij@gmail.com
Ресей, Moscow, 119071

D. Zykova

Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences; Moscow Institute of Physics and Technology (National Research University)

Email: sokolov.valerij@gmail.com
Ресей, Moscow, 119071; Dolgoprudny, Moscow oblast, 141700

L. Pozdeeva

Lomonosov Moscow State University

Email: sokolov.valerij@gmail.com
Ресей, Moscow, 119991

Әдебиет тізімі

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2. Fig. 1. Dependence of the difference in the boundary potentials of the BLM ∆φb on the concentration of pyranine C in the solution on one side of the membrane. The BLM was formed in a solution of 20 mM KCl, 2 mM Tris, HEPES and citrate, pH 6.0. The straight line is drawn according to equation (1) at z = 3.

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3. Fig. 2. Kinetics of changes in the boundary potential difference of the BLM upon addition of di-4-ANEPPS at a concentration of 6 μM (a) or RH-421 at a concentration of 7.5 μM (b) and pyranine at a concentration of 100 μM to the solution on one side of the membrane, as well as upon subsequent switching on (L) and switching off of the light (D). The BLM was formed in a solution of 20 mM KCl, 0.2 mM Tris, HEPES and citrate, pH 7. The moments of addition of di-4-ANEPPS, RH-421 and pyranine, as well as switching on and off of the light are shown by arrows.

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4. Fig. 3. Dependence of the rate of decrease of the dipole potential caused by the adsorption of di-4-ANEPPS on the concentration of pyranine C (a, illumination power 0.4 W), and on the illumination power P (b, pyranine concentration 10 μM). BLM was formed in a solution of 20 mM KCl, 0.2 mM Tris, HEPES and citrate, pH 7.0.

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5. Fig. 4. Dependence of the change in the boundary potential of BLM caused by the adsorption of pyranine on its concentration in a solution in which 5 μM di-4-ANEPPS is also present (white circles) and in the absence of di-4-ANEPPS (black circles). BLM was formed in a solution of 20 mM KCl, 2 mM Tris, HEPES and citrate, pH 7.0.

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6. Fig. 5. Dependence of the rate of potential disappearance upon illumination on the concentration of HEPES in the solution. BLM was formed in a solution of 20 mM KCl, 0.2 mM Tris, HEPES and citrate, pH 7.0. The concentration of pyranine was 10 μM, the illumination power was 0.2 W.

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