Diffraction optical elements for the implementation of three-dimensional nanoscopy using rotating light fields

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Diffraction optical elements, made by contact printing on a bichrome gelatin and direct laser recording using photoresist, have been studied in order to modify the point-scattering function for the implementation of 3D ultrahigh-resolution fluorescence microscopy. It has been shown that these elements produce two-lobed, rotating light fields that can be used for 3D nanoscopy. Results of 3D subdiffractive localization of fluorescent labels, with an assessment of the accuracy of coordinate restoration, have also been presented.

Full Text

Restricted Access

About the authors

D. V. Prokopova

Lebedev Physical Institute of the Russian Academy of Sciences

Author for correspondence.
Email: prokopovadv@lebedev.ru

Branch in Samara

Russian Federation, Samara

I. Y. Eremchev

Institute of Spectroscopy of the Russian Academy of Sciences; Lebedev Physical Institute of the Russian Academy of Sciences

Email: prokopovadv@lebedev.ru

Branch in Troitsk

Russian Federation, Moscow; Moscow

N. N. Losevsky

Lebedev Physical Institute of the Russian Academy of Sciences

Email: prokopovadv@lebedev.ru

Branch in Samara

Russian Federation, Samara

D. А. Belousov

Institute of Automation and Electrometry of the Siberian Branch of the Russian Academy of Sciences

Email: prokopovadv@lebedev.ru
Russian Federation, Novosibirsk

S. K. Golubtsov

Institute of Automation and Electrometry of the Siberian Branch of the Russian Academy of Sciences

Email: prokopovadv@lebedev.ru
Russian Federation, Novosibirsk

S. P. Kotova

Lebedev Physical Institute of the Russian Academy of Sciences

Email: prokopovadv@lebedev.ru

Branch in Samara

Russian Federation, Samara

А. V. Naumov

Lebedev Physical Institute of the Russian Academy of Sciences; Moscow State Pedagogical University

Email: prokopovadv@lebedev.ru

Branch in Troitsk

Russian Federation, Moscow; Moscow

References

  1. Mockl L., Moerner W.E. // J. Amer. Chem. Soc. 2020. V. 142. No. 42. P. 17811.
  2. Naumov A., Eremchev I. Yu., Gorshelev A.A. // Eur. Phys. J. D. 2014. V. 68. P. 348.
  3. Hettich C., Schmitt C., Zitzmann J. et al. // Science. 2002. V. 298. No. 5592. P. 385.
  4. Еремчев И.Ю., Лозинг Н.А., Баев А.А. и др. // Письма в ЖЭТФ. 2018. Т. 108. № 1. С. 26, Eremchev I.Y., Lozing N.A., Baev A.A. et al. // JETP Lett. 2018. V. 108. No. 1. P. 30.
  5. Осадько И.С. // Письма в ЖЭТФ. 2018. Т. 107. № 11. С. 755, Osad’ko I.S. // JETP Lett. 2018. V. 107. No. 11. P. 725.
  6. Naumov A.V., Gorshelev A.A., Gladush M.G. et al. // Nano Lett. 2018. V. 18. No. 10. P. 6129.
  7. Гладуш М.Г., Аникушина Т.А., Горшелев А.А. и др. // ЖЭТФ. 2019. Т. 155. № 5. С. 771, Gladush M.G., Anikushina T.A., Gorshelev A.A. et al. // JETP. 2019. V. 128. No. 5. P. 655.
  8. Moerner W.E. // Angew. Chem. Int. Edit. 2015. V. 54. No. 28 P. 8067.
  9. Betzig E. // Angew. Chem. Int. Edit. 2015. V. 54. No. 28. P. 8034.
  10. Hell S.H. // Angew. Chem. Int. Edit. 2015. V. 54. No. 28. P. 8054.
  11. Greengard A., Schechner Y., Piestun R. // Opt. Lett. 2006. V. 31. No. 2. P. 181.
  12. Huang B., Wang W., Bates M., Zhuang X. // Science. 2008. V. 319. No. 5864. P. 810.
  13. Shechtman Y., Sahl S.J., Backer A.S., Moerner W.E. // Phys. Rev. Lett. 2014. V. 113. No. 13. Art. No. 133902.
  14. Pavani S.R.P., Piestun R. // Opt. Express. 2008. V. 16. No. 5. P. 3484.
  15. Grover G., DeLuca K., Quirin S.et al. // Opt. Express. 2012. V. 20. No. 24. P. 26681.
  16. Baranek M., Bouchal Z. // JEOS-RP. 2013. V. 8. Art. No. 13017.
  17. Anand V., Khonina S., Kumar R. et al. // Nanoscale Res. Lett. 2022. V. 17. No. 1. P. 1.
  18. Прокопова Д.В., Котова С.П., Самагин С.А. // Изв. РАН. Сер. физ. 2021. Т. 85. № 8. С. 1205, Prokopova D.V., Kotova S.P., Samagin S.A. // Bull. Russ. Acad. Sci. Phys. 2021. V. 85. No. 8. P. 928.
  19. Волостников В.Г., Воронцов Е.Н., Котова С.П. и др. // Изв. вузов. Физика. 2015. Т. 58. № 11—3. С. 73.
  20. Прокопова Д.В., Воронцов Е.Н., Лосевский Н.Н., Разуева Е.В. // Изв. Самар. НЦ РАН. 2015. Т. 17. № 2. С. 97.
  21. Волостников В.Г., Воронцов Е.Н., Котова С.П. и др. // Изв. РАН. Сер. физ. 2016. Т. 80. № 7. С. 841, Volostnikov V.G., Vorontsov E.N., Kotova S.P. et al. // Bull. Russ. Acad. Sci. Phys. 2016. V. 80. No. 7. P. 766.
  22. Воронцов Е.Н., Лосевский Н.Н., Прокопова Д.В., и др. // Комп. оптика. 2016. Т. 40. № 2. С. 158.
  23. Прокопова Д.В., Воронцов Е.Н., Котова С.П. и др. // Изв. РАН. Сер. физ. 2019. Т. 83. № 12. С. 1612, Prokopova D.V., Vorontsov E.N., Kotova S.P. et al. // Bull. Russ. Acad. Sci. Phys. 2019. V. 83. No. 12. P. 1453.
  24. Еремчев И.Ю., Прокопова Д.В., Лосевский Н.Н. и др. // УФН. 2022. Т. 192. № 6. С. 663, Eremchev I.Y., Prokopova D.V., Losevskii N.N. et al. // Phys. Usp. 2022. V. 65. No. 6. P. 617.
  25. Abramochkin E., Volostnikov V. // Opt. Commun. 1993. V. 102. No. 3—4. P. 336.
  26. Абрамочкин Е.Г., Волостников В.Г. // УФН. 2004. Т. 174. № 12. С. 1273, Abramochkin E.G., Volostnikov V.G. // Phys. Usp. 2004. V. 47. No. 12. P. 1177.
  27. Абрамочкин Е.Г., Волостников В.Г. Современная оптика гауссовых пучков. М.: Физматлит, 2010. 184 с.
  28. https://www.hamamatsu.com/us/en/product/optical-components/lcos-slm/priciple.html.
  29. Воронцов Е.Н., Котова С.П., Лосевский Н.Н. и др. // Кратк. сообщ. по физ. ФИАН. 2018. Т. 45. № 3. С. 9, Vorontsov E.N., Kotova S.P., Losevsky N.N. et al. // Bull. Lebedev Phys. Inst. 2018. V. 45. No. 3. P. 71.
  30. Прокопова Д.В., Котова С.П. // Фотоника. 2020. Т. 14. № 2. С. 170, Prokopova D.V., Kotova S.P. // Photonics Russ. V. 14. No. 2. P. 170.
  31. Korolkov V.P., Pruss C., Reichelt S., Tiziani H.J. // Proc. SPIE. 2002. V. 4900. P. 873.
  32. Korolkov V.P., Nasyrov R.K., Shimansky R.V. // Appl. Optics. 2006. V. 45. No. 1. P. 53.
  33. Xie S., Erjawetz J., Schuster C., Schift H. // J. Vac. Sci. Technol. B. 2021. V. 39. No. 5. Art. No. 052603.
  34. https://heidelberg-instruments.com/product/dwl-66-laser-lithography-system.
  35. Баев А.А., Роженцов А.А., Наумов А.В., Еремчев И.Ю. Программа для распознавания двулепестковых изображений люминесцирующих наночастиц для трехмерной микроскопии сверхвысокого разрешения. Свид. о гос. рег. прогр. для ЭВМ № 2022611212, 2022.
  36. Еремчев И.Ю., Лозинг Н.А., Гладуш М.Г. и др. // Изв. РАН. Сер. физ. 2018. Т. 82. № 11. С. 1624, Eremchev I.Y., Lozing N.A., Gladush M.G. et al. // Bull. Russ. Acad. Sci. Phys. 2018. V. 82. No. 11. P. 1482.
  37. Gritchenko A.S., Eremchev I.Y., Naumov A.V., et al. // Opt. Laser Technol. 2021. V. 143. Art. No. 107301.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. Phase function of the DOE optimized for operation in a three-dimensional nanoscope (a). Intensity distributions formed by the DOE manufactured by direct laser writing on photoresist at different distances near the focal plane of a lens with F = 250 mm (b). The frame side size is 2 mm.

Download (164KB)
Indexing metadata
3. Fig. 2. Installation diagram of a three-dimensional nanoscope with a stationary reflective DOE.

Download (403KB)
Indexing metadata
4. Fig. 3. Two-lobe images of point fluorescent labels in a 3-D nanoscope with a stationary DOE; for different positions of the emitters relative to the focal plane of the microscope δz: −2 (a1); −1 (a2); 0 (a3) ​​and 1.5 μm (a4). Dependence of the rotation angle of the two-lobe image α on the distance δz (b). Dots are experimentally measured values; the red line is a linear approximation of the dependence.

Download (345KB)
Indexing metadata
5. Fig. 4. Accuracy of axial coordinate reconstruction. Dependence of the accuracy of longitudinal coordinate reconstruction of point fluorescent labels on the number of registered photons N for the tilt angle α ~ 0° (a). The inset shows an example of the distribution of reconstructed longitudinal coordinates z; obtained in a series of measurements n = 200 and its approximation by the Gaussian function. Dependence of the longitudinal coordinate reconstruction error σz(α) on the image rotation angle for a fixed number of photons N (b).

Download (240KB)
Indexing metadata

Copyright (c) 2024 Russian Academy of Sciences