Catalytic properties of a nanozyme based on silver nanoparticles immobilized in a polymethacrylate matrix

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Abstract

This article presents studies on the catalytic, peroxidase-like properties of silver nanoparticles (Ag NPs) immobilized in polymethacrylate matrix (PMM). Ag NPs were prepared by thermal reduction of silver cations pre-immobilized in PMM. The morphology of the nanocomposite was studied using scanning electron microscopy, and the average size of the synthesized individual spherical nanoparticles was 18 ± 5 nm. It was demonstrated that silver nanoparticles immobilized in a polymethacrylate matrix (PMM-Ag0) exhibit pronounced peroxidase-like activity in the oxidation reaction of the chromogenic substrate – indigocarmine in the presence of H₂O₂. The Michaelis–Menten model was used to assess the kinetic parameters of the reaction. The values of Michaelis constant (Km) observed for indigocarmine and H₂O₂ (0.1 mM and 1.0 mM, respectively) show strong affinity of the substrates to silver nanoparticles in PMM.

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About the authors

S. K. Bragina

National Research Tomsk State University

Author for correspondence.
Email: braginask@gmail.com
Russian Federation, Lenin Ave., 36, Tomsk, 634050

N. A. Gavrilenko

National Research Tomsk State University

Email: braginask@gmail.com
Russian Federation, Lenin Ave., 36, Tomsk, 634050

N. V. Saranchina

National Research Tomsk State University

Email: braginask@gmail.com
Russian Federation, Lenin Ave., 36, Tomsk, 634050

M. A. Gavrilenko

National Research Tomsk Polytechnic University

Email: braginask@gmail.com
Russian Federation, Lenin Ave., 30, Tomsk, 634050

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Supplementary files

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2. Fig. 1. Absorption spectra of PMM-Ag0 at different Ag contents in PMM depending on the contact time with AgNO3 solution: 1 – 4 min (aAg = 0.56 wt.%); 2 – 3 min (aAg = 0.29 wt.%); 3 – 2 min (aAg = 0.17 wt.%).

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3. Fig. 2. Absorption spectra of PMM-Ag0 at different storage times.

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4. Fig. 3. SEM images of Ag nanoparticles in PMM obtained by thermal reduction, at scales of 2 μm (a) and 400 nm (b).

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5. Fig. 4. Size distribution of Ag nanoparticles in PMM.

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6. Fig. 5. Photograph and absorption spectra of solutions: 1 – indigo carmine, 2 – indigo carmine + H2O2, 3 – indigo carmine + PMM-Ag0, 4 – indigo carmine + H2O2 + PMM-Ag0. Cindigo carmine = 2.2 × 10–4 M, CH2O2 = 2.7 × 10–3 M, pH 4.0, reaction time – 50 min.

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7. Fig. 6. Dependence of indigo carmine conversion (ΔA/A0) on reaction conditions: a – Ag nanoparticle content in PMM, b – pH value, c – temperature. Reaction conditions: Cindigo carmine = 2.2 × 10–4 M, CH2O2 = 2.7 × 10–3 M.

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8. Fig. 7. Kinetic curves of indigo carmine concentration oxidized by H2O2 in the presence of PMM-Ag0 as a function of time: a – at an initial indigo carmine concentration of 9.5 × 10–4 M and initial H2O2 concentrations of 1.6 × 10–4 (1), 5.3 × 10–4 (2), 1.6 × 10–3 (3), 2.7 × 10–3 (4), and 3.7 × 10–3 M (5); b – at an initial H2O2 concentration of 2.6 × 10–3 M and initial indigo carmine concentrations of 2.0 × 10–5 (1), 3.9 × 10–5 (2), 1.1 × 10–4 (3), 1.9 × 10–4 (4), and 2.6 × 10–4 M (5).

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9. Fig. 8. Curves of initial rate (V0) of indigo carmine oxidation in the presence of PMM-Ag0 versus initial concentrations of H2O2 (a) and indigo carmine (b) in the studied solutions. Graphs of V0 of indigo carmine oxidation in the presence of PMM-Ag0 versus initial concentrations of H2O2 (c) and indigo carmine (d) in Lineweaver–Burk coordinates.

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10. Oxidation of indigo carmine

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