Email this article 
Development of N.K. Koltsov Idea about Genetic Organization of Interbands in Drosophila melanogaster Polytene Chromosomes
- Authors: Zhimulev I.F.1, Vatolina T.Y.1, Levitsky V.G.2, Kolesnikova T.D.1, Tsukanov A.V.2
-
Affiliations:
- Institute of Molecular and Cellular Biology SB RAS
- Institute of Cytology and Genetics SB RAS
- Issue: Vol 54, No 2 (2023)
- Pages: 172-175
- Section: Short communications
- URL: https://ruspoj.com/0475-1450/article/view/669950
- DOI: https://doi.org/10.31857/S0475145023020064
- EDN: https://elibrary.ru/XFKXAP
- ID: 669950
Cite item
Open Access
Access granted
Subscription Access
Abstract
Here for the first time, the organization of promoters of developmental gene promoters and promoter of genes necessary for general cellular functions—the “houskeeping” of the cell in the complete genome of Drosophila melanogaster were studied. Using bioinformatic methods, it has been shown that the genes whose promoters are located in the interbands of polytene chromosomes are enriched in functions associated with general cellular processes, while the rest of the genes (about half of the Drosophila genome) are associated with highly specialized processes occurring during development. In the promoter zone of the housekeeping genes, four specific motifs were found that can be present in different genes individually or in various combinations. A significant part of interband promoters do not contain identified motifs. The analysis carried out using Gene Ontology showed that certain groups of interband genes containing one motif in promoters or their combinations are characterized by the performance of certain functions.
About the authors
I. F. Zhimulev
Institute of Molecular and Cellular Biology SB RAS
Author for correspondence.
Email: zhimulev@mcb.nsc.ru
Russia, 630090, Novosibirsk, prosp. ak. Lavrentieva 8/2
T. Yu. Vatolina
Institute of Molecular and Cellular Biology SB RAS
Email: zhimulev@mcb.nsc.ru
Russia, 630090, Novosibirsk, prosp. ak. Lavrentieva 8/2
V. G. Levitsky
Institute of Cytology and Genetics SB RAS
Email: zhimulev@mcb.nsc.ru
Russia, 630090, Novosibirsk, prosp. ak. Lavrentieva 10
T. D. Kolesnikova
Institute of Molecular and Cellular Biology SB RAS
Email: zhimulev@mcb.nsc.ru
Russia, 630090, Novosibirsk, prosp. ak. Lavrentieva 8/2
A. V. Tsukanov
Institute of Cytology and Genetics SB RAS
Email: zhimulev@mcb.nsc.ru
Russia, 630090, Novosibirsk, prosp. ak. Lavrentieva 10
References
- Andersson R., Sandelin A. Determinants of enhancer and promoter activities of regulatory elements // Nat. Rev. Genet. 2020. V. 21. P. 71–87.
- Crick F. General model for the chromosomes of higher organisms // Nature. 1971. V. 234. P. 25–27.
- Danino Y.M., Even D., Ideses D. et al. The core promoter: At the heart of gene expression // Biochim. Biophys. Acta. 2015. V. 1849(8). P. 1116–1131.
- Koltzoff N.K. The structure of the chromosomes in the salivary glands of Drosophila // Science. 1934. V. 60. P. 312–313.
- Lenhard B., Sandelin A., Carninci P. Metazoan promoters: emerging characteristics and insights into transcriptional regulation // Nat. Rev. Genet. 2012. V. 13(4). P. 233–245.
- Levitsky V.G., Zykova T.Yu., Moshkin Y.M., Zhimulev I.F. Nucleosome positioning around transcription start site correlates with gene expression only for active chromatin state in Drosophila interphase chromosomes // International J. Molecular Sciences. 2020. V. 21(23). P. 9282.
- Paul J. General theory of chromosomes structures and gene activation in eukaryotes // Nature. 1972. V. 238. P. 444–446.
- Ramalingam V., Natarajan M., Johnston J. et al. TATA and paused promoters active in differentiated tissues have distinct expression characteristics // Mol. Syst. Biol. 2021. V. 17. P. e9866.
- Zhimulev I.F., Belyaeva E.S. Proposals to the problem of structural and functional organization of polytene chromosomes // Theor. Appl. Genet. 1975. V. 45(8). P. 335–340.
- Zhimulev I.F., Zykova T.Yu., Goncharov F.P. et al. Genetic organization of polytene chromosome bands and interbands in Drosophila melanogaster // PLoS One. 2014. V. 9(7). P. e101631.
- Zykova T.Yu., Levitsky V.G., Belyaeva E.S. et al. Polytene chromosomes – a portrait of functional organization of the Drosophila genome // Curr. Genomics. 2018. V. 19(3). P. 179–191.
Supplementary files
