Generation of a Null Mutation of the  toothrin  Gene by Targeted Homologous Recombination in  Drosophila melanogaster

E. E. Kuvaeva; Куваева Е. Е.; D. A. Kulikova; Куликова Д. А.; O. B. Simonova; Симонова О. Б.; I. B. Mertsalov; Мерцалов И. Б.

doi:10.31857/S0475145023030059

Generation of a Null Mutation of the toothrin Gene by Targeted Homologous Recombination in Drosophila melanogaster

Авторлар: Kuvaeva E.E.¹, Kulikova D.A.¹, Simonova O.B.¹, Mertsalov I.B.¹
Мекемелер:
1. Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Шығарылым: Том 54, № 3 (2023)
Беттер: 232-238
Бөлім: Short communications
URL: https://ruspoj.com/0475-1450/article/view/669940
DOI: https://doi.org/10.31857/S0475145023030059
EDN: https://elibrary.ru/ZRSGJG
ID: 669940

Дәйексөз келтіру

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Ашық рұқсат
Рұқсат жабық

Рұқсат берілді
Рұқсат жабық

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Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

Drosophila toothrin gene is related to the conserved d4 family of genes that encode specific transcription factors. In early works, we investigated the effect of tth gene overexpression and characterized the specific pattern of its expression in the nervous system, but mutations in the tth gene have not yet been found. Null mutants are essential for analyzing gene function and screening of partner genes. Here we report the generation of the first tth gene knockout in Drosophila using a homologous recombination technique.

Негізгі сөздер

d4 gene family, toothrin, gene knockout, Drosophila melanogaster

Әдебиет тізімі

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