Enviar artigo por via de e-mail Generation of a Null Mutation of the toothrin Gene by Targeted Homologous Recombination in Drosophila melanogaster
- Autores: Kuvaeva E.E.1, Kulikova D.A.1, Simonova O.B.1, Mertsalov I.B.1
-
Afiliações:
- Koltzov Institute of Developmental Biology of Russian Academy of Sciences
- Edição: Volume 54, Nº 3 (2023)
- Páginas: 232-238
- Seção: Short communications
- URL: https://ruspoj.com/0475-1450/article/view/669940
- DOI: https://doi.org/10.31857/S0475145023030059
- EDN: https://elibrary.ru/ZRSGJG
- ID: 669940
Citar
Acesso aberto
Acesso está concedido
Somente assinantes
Resumo
Drosophila toothrin gene is related to the conserved d4 family of genes that encode specific transcription factors. In early works, we investigated the effect of tth gene overexpression and characterized the specific pattern of its expression in the nervous system, but mutations in the tth gene have not yet been found. Null mutants are essential for analyzing gene function and screening of partner genes. Here we report the generation of the first tth gene knockout in Drosophila using a homologous recombination technique.
Palavras-chave
Sobre autores
E. Kuvaeva
Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Email: osimonova@hotmail.com
Russia, 119334, Moscow, ul. Vavilova 26
D. Kulikova
Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Email: osimonova@hotmail.com
Russia, 119334, Moscow, ul. Vavilova 26
O. Simonova
Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Autor responsável pela correspondência
Email: osimonova@hotmail.com
Russia, 119334, Moscow, ul. Vavilova 26
I. Mertsalov
Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Email: osimonova@hotmail.com
Russia, 119334, Moscow, ul. Vavilova 26
Bibliografia
- Симонова О.Б., Куликова Д.А., Мерцалов И.Б. и др. Исследование суперэкспрессии нового гена toothrin у дрозофилы // Генетика. 2005. Т. 41. № 2. С. 196–202.
- Ashburner M., Golic K.G., Hawley R.S. Drosophila: A Laboratory Handbook. Cold Spring Harbor Laboratory Press, 2005. 1409 p.
- Buchman V.L., Ninkina N.N., Bogdanov et al. Differential splicing creates a diversity of transcripts from a neurospecific developmentally regulated gene encoding a protein with new zinc-finger motifs // Nucleic Acids Res. 1992. V. 20(21). P. 5579–5585.
- Gong W.J., Golic K.G. Ends-out, or replacement, gene targeting in Drosophila // PNAS. 2003. V. 100(5). P. 2556–2561.
- Ishizaka A., Mizutani T., Kobayashi K. et al. Double plant homeodomain (PHD) finger proteins DPF3a and -3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF-κB RelA/p50 heterodimer // J. Biol. Chem. 2012. V. 287(15). P. 11924–11933.
- Kulikova D.A., Mertsalov I.B., Simonova O.B. d4 family genes: genomic organization and expression // Russ. J. Dev. Biol. 2013. V. 44. P. 1–6.
- Kuvaeva E.E., Kulikova D.A., Simonova O.B., Mertsalov I.B. Studying the specific localization of Toothrin protein from related D4 family in Drosophila melanogaster // Rus. J. Dev. Biol. 2022. V. 53. № 2. P. 145–149.
- Nabirochkina E.N., Simonova O.B., Mertsalov I.B. et al. Expression pattern of dd4, a sole member of the d4 family of transcription factors in Drosophila melanogaster // Mech. Dev. 2002. V. 114. P. 119–123.
- Nefedova L.N. Drosophila melanogaster as a model of developmental genetics: modern approaches and prospects // Russ. J. Dev. Biol. 2020. V. 51. P. 201–211.
- Turan S., Galla M., Ernst E. et al. Recombinase-mediated cassette exchange (RMCE): traditional concepts and current challenges // J. Mol. Biol. 2011. V. 407(2). P. 193–221.
- Will E., Klump H., Heffner N. et al. Unmodified Cre recombinase crosses the membrane // Nucleic Acids Res. 2002. V. 30(12):e59.
Arquivos suplementares
