REDUCTION OF NEPHROTOXICITY BY PERITONEAL DIALYSIS WITH ADDITION OF SODIUM HYDROCARBONATE DURING POISONING WITH URANIUM ACETATE DIHYDRATE IN RAT

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Abstract

The article presents the results of an experimental study on rats’ poisoning with uranyl acetate dihydrate (1 DL50). The aim of this work was to investigate the effectiveness to the sodium bicarbonate additive to the composition of the solution for peritoneal dialysis in relation to uranium clearance and survival of rats. The procedure of peritoneal dialysis was carried out in the “acute” mode in 1 day for 5 hours. Each rat received a total of 6 abdominal fillings and 6 ultrafiltration drains. The differences for pH Δ=1.71, EC Δ=5055 µS / cm and TDS Δ=2527 mg / L have been observed by addition of sodium bicarbonate (B, 1.5%) to the standard solution. The administration of solution B led to an increase in the rate of peritoneal clearance of uranium PCl 238U by 276.2% (p=0.0286, MannWhitney criterion) compared to standard solution A without sodium bicarbonate. The dynamics of ultrafiltration were characterized by a linear growth from 1 to 3 sessions (the slope of the linear regression is 70°) to an exit to the plateau by 3 sessions. The URR was 65.4% in the 1st day, 65% in the 3rd day, and 69.1% in the 7th day. The results of the Mantel-Cox test have showed that peritoneal dialysis with the addition of sodium bicarbonate as an antidote (solution B) contributed to a significant increase in postdialysis survival of rats (p=0.0018 compared to the negative control group, p=0.0425 compared to dialysis solution A).

About the authors

K. V. Sivak

A.A. Smorodintsev Research Institute of Influenza, Ministry of Healthcare of the Russian Federation

Author for correspondence.
Email: kvsivak@gmail.com
197376, Saint Petersburg Russian Federation

T. N. Savateeva-Lyubimova

A.A. Smorodintsev Research Institute of Influenza, Ministry of Healthcare of the Russian Federation

Email: drugs_safety@mail.ru
197376, Saint Petersburg Russian Federation

T. A. Gus’kova

M.V. Dorogov Yaroslavl Center for the Transfer of Pharmaceutical Technologies, K.D. Ushinsky Yaroslavl State Pedagogical University

Email: tagus@rambler.ru
150010, Yaroslavl Russian Federation

R. G. Guseinov

Clinical Hospital of St. Luka (Saint Petersburg Clinical Hospital of St. Luka)

Email: rusfa@yandex.ru
194044, Saint Petersburg Russian Federation

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Copyright (c) 2019 Sivak K.V., Savateeva-Lyubimova T.N., Gus’kova T.A., Guseinov R.G.


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