EVALUATION OF CYTOTOXICITY AND GENOTOXICITY OF NEW SUBSTITUTED PIRYDO [1,2-A] BENZIMIDAZOLES USING THE ALLIUM TEST

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Abstract

Investigations were carried out into mutagenic and mitosis modifying action (Allium test) of imidazole new polycyclic condensed derivatives having a high ability to embed into DNA molecules: 7-trifluor methyl pyrido [1,2- a] benzimidazole, 7- nitropyrido[1,2-a] benzimidazole, 7- amino pyrido [1,2- a] benzimidazole, 8-nitro- 7-trifluor methyl pyrido[1,2-a]benzimidazole, 8-amino- 7-trifluor methyl pyrido[1,2-a] benzimidazole. The evaluation of cytotoxic effect of compounds under investigation was performed by comparing with toxicological characteristics of a widely used DNA –intercalator 9-amino acridine. All azagetero cycles induced reduction of the prophase index evidencing their impact on the process of DNA replication. The most wide spread mutations were “bridge” type pathologies as a result of reunion of different daughter cells chromosomes and obliteration and losses of entire chromosomes at cells division. All mono- and di-substituted pirido[1,2-a] benzimidazoles produced a lesser mitodepressive and mutagenic action than 9-amino acridine. Monosubstituted pirido[1,2-a]benzimidazoles had lesser cytotoxicity and mutagenicity in the A cepa test-system.

About the authors

Roman Sergeevich Begunov

Federal State Budgetary Educational Institution of Additional Higher Education «P.G. Demidov Yaroslavl State University»

Author for correspondence.
Email: noemail@neicon.ru

Кандидат химических наук, доцент, кафедра органической и биологической химии факультета биологии и экологии ФГБОУ ВПО «Ярославский государственный университет им. П.Г. Демидова», г. Ярославль

e-mail: begunov@bio.uniyar.ac.ru

Russian Federation

Alexander Andreevich Sokolov

Federal State Budgetary Educational Institution of Additional Higher Education «P.G. Demidov Yaroslavl State University»

Email: noemail@neicon.ru

Аспирант второго года обучения, специальности 02.00.03, факультет биологии и экологии ФГБОУ ВПО «Ярославский государственный университет им. П.Г. Демидова», г. Ярославль

e-mail: morose@mail.ru

Russian Federation

Tatyana Viktorovna Shebunina

Federal State Budgetary Educational Institution of Additional Higher Education «P.G. Demidov Yaroslavl State University»

Email: noemail@neicon.ru

Магистрант первого года обучения, специальность Химия, факультет биологии и экологии ФГБОУ ВПО «Ярославский государственный университет им. П.Г. Демидова», г. Ярославль

e-mail: tan4ik2107@mail.ru

Russian Federation

Svetlana Alexandrovna Kalina

Federal State Budgetary Educational Institution of Additional Higher Education «P.G. Demidov Yaroslavl State University»

Email: noemail@neicon.ru

Студентка 3 курса, специальность Химия, факультет биологии и экологии ФГБОУ ВПО «Ярославский государственный университет им. П.Г. Демидова», г. Ярославль

e-mail: svetlana_kalina1993@mail.ru

Russian Federation

Alexandra Alexandrovna Bashkirova

Federal State Budgetary Educational Institution of Additional Higher Education «P.G. Demidov Yaroslavl State University»

Email: noemail@neicon.ru

Студентка 3 курса, специальность Химия, факультет биологии и экологии ФГБОУ ВПО «Ярославский государственный университет им. П.Г. Демидова», г. Ярославль

e-mail: yarchemlab@gmail.com

Russian Federation

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Copyright (c) 2015 Begunov R.S., Sokolov A.A., Shebunina T.V., Kalina S.A., Bashkirova A.A.


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