ACTIVITY OF APOPTOSIS IN THE NERVE TISSUE OF WHITE RATS EXPOSED TO NANOSILVER ARABINOGALACTAN

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Abstract

The article presents results of apoptosis evaluation in albino rat neurons using comet assay for DNA and immune histochemical analysis to determine the activity of pro-and anti-apoptotic proteins caspase 3 and bcl-2 at exposure to nano biocomposites representing nano silver embedded in a matrix of a natural arabinogalactan. In preparations of nervous tissue of rats exposed to nano silver arabinogalactan polymer, a statistically significant increase of DNA content in comet tails was found out. DNA comets with DNA tail content of more than 70%, which could be regarded as apoptotic have not been observed in investigated formulations. The results of the immune histochemical determination of expression of anti-and pro- apoptotic proteins bcl-2 and caspase-3 in neurons show on the contrary the development of apoptosis in nerve cells. Comparing results of the studies performed, a conclusion can be drawn that to detect the apoptosis process, immune histochemical methods for determination of anti-and pro-apoptotic proteins are more efficient than DNA comet assay.

About the authors

Larisa Mikhailovna Sosedova

East-Siberian Institute of Medico-Ecological Researches

Author for correspondence.
Email: noemail@neicon.ru

Д.м.н., проф., зав.лабораторией биомоделирования и трансляционной медицины ФГБНУ «Восточно-Сибирский институт медико-экологических исследований», 665827, г. Ангарск, Российская Федерация,

e-mail: sosedlar@mail.ru

Russian Federation

Ekaterina Aleksandrovna Kapustina

East-Siberian Institute of Medico-Ecological Researches

Email: noemail@neicon.ru

К.м.н., научный сотрудник лаборатории биомоделирования и трансляционной медицины ФГБНУ «Восточно-Сибирский институт медико-экологических исследований», 665827, г. Ангарск, Российская Федерация

e-mail: tox_lab@mail.ru 

Russian Federation

Mikhail Aleksandrovich Novikov

East-Siberian Institute of Medico-Ecological Researches

Email: noemail@neicon.ru

М.н.с., лаборатории биомоделирования и трансляционной медицины ФГБНУ «Восточно-Сибирский институт медико-экологических исследований», 665827, г. Ангарск, Российская Федерация

e-mail: novik-imt@mail.ru

Russian Federation

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Copyright (c) 2015 Sosedova L.M., Kapustina E.A., Novikov M.A.


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