Cytokine levels in individuals occupationally exposed to ionising radiation

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Abstract

Cytokines are proteins produced by various cells of the body and are intercellular messengers. They perform many functions that are very important for understanding the pathogenesis of early and late effects of exposure, their prevention and treatment. The purpose of this work was to study the cytokine profile in individuals exposed to chronic occupational exposure. The main group consisted of employees of the Mayak nuclear industry enterprise who were exposed to chronic exposure as a result of their professional activities. The control group consisted of residents of the city of Ozyersk, Chelyabinsk region, who were not exposed to chronic exposure as a result of their professional activities. The study used enzyme immunoassay, which was carried out in accordance with the instructions of the manufacturers of the test systems. Statistical analysis of the obtained data was carried out using the Mann–Whitney method. Serum levels of IFNγ and TNFα were increased in the occupationally exposed group. In addition, it was found that the content of IL-18 и IL-35 in blood serum increased with an increase in the dose of internal α-irradiation to the red bone marrow (RBM), and the concentration of IL-17А, IL-35 и TNFα – with an increase in the dose of external irradiation on the RBM. External γ-irradiation suppressed the expression of IL-27 in the blood serum of workers. The content of anti-inflammatory cytokines in the blood serum of exposed individuals was not changed. The results obtained allow us to conclude that the expression profile of the studied cytokines was shifted to the inflammatory side in the long-term period after the end of occupational exposure.

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About the authors

Valentina L. Rybkina

South Ural Biophysics Institute affiliated to the Federal Medical Biological Agency

Email: clinic@subi.su
ORCID iD: 0000-0001-5096-9774
Russian Federation, Ozyersk

Darya S. Oslina

South Ural Biophysics Institute affiliated to the Federal Medical Biological Agency

Author for correspondence.
Email: clinic@subi.su
ORCID iD: 0000-0003-4757-7969
Russian Federation, Ozyersk

Tamara V. Azizova

South Ural Biophysics Institute affiliated to the Federal Medical Biological Agency

Email: clinic@subi.su
ORCID iD: 0000-0001-6954-2674
Russian Federation, Ozyersk

Elena D. Drugova

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation

Email: clinic@subi.su
ORCID iD: 0000-0002-9304-7371
Russian Federation, Moscow

Galina V. Adamova

South Ural Biophysics Institute affiliated to the Federal Medical Biological Agency

Email: clinic@subi.su
ORCID iD: 0000-0002-8776-4104
Russian Federation, Ozyersk

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2. Fig. 1. Levels of IL-2 in blood serum of the comparison group in relation to age

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3. Fig. 2. Levels of IL-17A in blood serum of Mayak PAworkers in relation to the cumulative bone marrow absorbed dose from external γ-exposure

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4. Fig. 3. Levels of IL-35 in blood serum of Mayak PAworkers in relation to the cumulative bone marrow absorbed dose from external γ-exposure.

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5. Fig. 4. Levels of TNFα in blood serum of Mayak PAworkers in relation to the cumulative bone marrow absorbed dose from external γ-exposure.

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6. Fig. 5. Levels of IL-18 in blood serum of Mayak PAworkers in relation to the cumulative bone marrow absorbed dose from internal α-exposure.

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7. Fig. 6. Levels of IL-35 in blood serum of Mayak PAworkers in relation to the cumulative bone marrow absorbed dose from internal α-exposure.

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8. Fig. 7. Levels of IL-27 in blood serum of Mayak PAworkers in relation to the cumulative bone marrow absorbed dose from internal α-exposure.

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