Control levels of tranlocations in human peripheral blood lymphocyte cultures determined using three-color FISH-staining

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Abstract

This paper presents the results of using three-color FISH-staining of chromosomes (DNA probes for chromosome pairs 1, 4, and 12) to study the control level of translocations in peripheral blood lymphocyte cultures of 35 relatively healthy volunteer donors aged from 21 to 72 years. Such information is necessary for the retrospective establishment of both the fact of overexposure itself and for an adequate assessment of the dose received. Translocations made up the bulk of the recorded colored rearrangements. Their frequency was significantly 3.3 times higher than the level of unstable aberrations (dicentrics and acentrics). At the same time, a moderate correlation was observed between the level of FISH translocations and the age of the donors, and gender did not affect this indicator in any way. Also, no significant effect of various types of medical diagnostic radiation, smoking and the disease of the new coronavirus infection COVID-19 on the studied index of chromosome damage was found. In general, the detected frequencies of translocations in genome-equivalent cells in their relationship with age were in fairly good agreement with the results of international studies.

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About the authors

Elena E. Lomonosova

State Research Center – Burnasyan Federal Medical Biophysical Center of Federal medical Biological Agency

Email: mrs.lomonosova@gmail.com
ORCID iD: 0000-0003-3739-4261
Russian Federation, Moscow

Vladimir Y. Nugis

State Research Center – Burnasyan Federal Medical Biophysical Center of Federal medical Biological Agency

Author for correspondence.
Email: nugisvju@list.ru
ORCID iD: 0000-0001-7396-0246
Russian Federation, Moscow

Maria G. Kozlova

State Research Center – Burnasyan Federal Medical Biophysical Center of Federal medical Biological Agency

Email: koslovaamg@yandex.ru
Russian Federation, Moscow

Victoria A. Nikitina

State Research Center – Burnasyan Federal Medical Biophysical Center of Federal medical Biological Agency

Email: nikitinava@yandex.ru
ORCID iD: 0000-0002-3150-7686
Russian Federation, Moscow

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2. Fig. 1. Dependence graph (solid line) with 95% confidence intervals (dashed lines) of the real frequencies of FISH-detected translocations on the age of relatively healthy donors.

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3. Fig. 2. Dependences of translocation frequencies per 100 genome-equivalent cells on age. The data taken from the article [18] are highlighted by shaded circles and solid lines: averages with 95% confidence intervals for 5 years and a linear regression constructed for them (the 95% confidence zone of the regression is indicated by a dotted line). Squares and pointed lines indicate averages with 95% confidence intervals obtained in this work (see text).

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