Assessment of Anticholinergic and Antidiabetic Properties of Some Natural and Synthetic Molecules: An In vitro and In silico Approach


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Abstract

Introduction:This study aimed to determine the in vitro and in silico effects of some natural and synthetic molecules on acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and α-glucosidase enzymes.

Background:Alzheimer's disease (AD) and Type II diabetes mellitus (T2DM) are considered the most important diseases of today’s world. However, the side effects of therapeutic agents used in both diseases limit their use. Therefore, developing drugs with high therapeutic efficacy and better pharmacological profile is important.

Objective:This study sets out to determine the related enzyme inhibitors used in treating AD and T2DM, considered amongst the most important diseases of today’s world.

Methods:In the current study, the in vitro and in silico effects of dienestrol, hesperetin, Lthyroxine, 3,3',5-Triiodo-L-thyronine (T3) and dobutamine molecules on AChE, BChE and α- glycosidase enzyme activities were investigated.

Results:All the molecules showed an inhibitory effect on the enzymes. The IC50 and Ki values of the L-Thyroxine molecule, which showed the strongest inhibition effect for the AChE enzyme, were determined as 1.71 µM and 0.83 ± 0.195 µM, respectively. In addition, dienestrol, T3, and dobutamine molecules showed a more substantial inhibition effect than tacrine. The dobutamine molecule showed the most substantial inhibition effect for the BChE enzyme, and IC50 and Ki values were determined as 1.83 µM and 0.845 ± 0.143 µM, respectively. The IC50 and Ki values for the hesperetin molecule, which showed the strongest inhibition for the α-glycosidase enzyme, were determined as 13.57 µM and 12.33 ± 2.57 µM, respectively.

Conclusion:According to the results obtained, the molecules used in the study may be considered potential inhibitor candidates for AChE, BChE and α-glycosidase.

About the authors

Veysel Çomaklı

Department of Nutrition and Dietetics, İbrahim Çeçen University of Ağrı

Author for correspondence.
Email: info@benthamscience.net

İmdat Aygül

Department of Nutrition and Dietetics,, Gümüşhane University

Email: info@benthamscience.net

Rüya Sağlamtaş

Department of Medical Services and Techniques,, İbrahim Çeçen University of Ağrı,

Email: info@benthamscience.net

Müslüm Kuzu

Department of NutritioDepartment of Nutrition and Dieteticsn and Dietetics, Karabük University

Email: info@benthamscience.net

Ramazan Demirdağ

Department of Nutrition and Dietetics, İbrahim Çeçen University of Ağrı

Email: info@benthamscience.net

Hülya Akincioğlu

Department of Chemistry, İbrahim Çeçen University of Ağrı

Email: info@benthamscience.net

Şevki Adem

Department of Chemistry, Çankırı Karatekin University

Email: info@benthamscience.net

İlhami Gülçin

Department of Chemistry, Atatürk University

Email: info@benthamscience.net

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