The Diagnostic Features of Peripheral Blood Biomarkers in Identifying Osteoarthritis Individuals: Machine Learning Strategies and Clinical Evidence

  • Authors: Zhou Q.1, Liu J.2, Xin L.3, Hu Y.4, Qi Y.3
  • Affiliations:
    1. Department of Rheumatism Immunity,, The First Affiliated Hospital of Anhui University of Chinese Medicine,
    2. Department of Rheumatism Immunity, Anhui University of Chinese Medicine
    3. Institute of Rheumatism Prevention and Treatment of Traditional Chinese Medicine, Anhui Academy of Chinese Medicine Sciences
    4. Institute of Rheumatism Prevention and Treatment of Traditional Chinese Medicine,, Anhui Academy of Chinese Medicine Sciences
  • Issue: Vol 20, No 6 (2024)
  • Pages: 928-942
  • Section: Chemistry
  • URL: https://ruspoj.com/1573-4099/article/view/644420
  • DOI: https://doi.org/10.2174/1573409920666230818092427
  • ID: 644420

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Abstract

Background:People with osteoarthritis place a huge burden on society. Early diagnosis is essential to prevent disease progression and to select the best treatment strategy more effectively. In this study, the aim was to examine the diagnostic features and clinical value of peripheral blood biomarkers for osteoarthritis.

Objective:The goal of this project was to investigate the diagnostic features of peripheral blood and immune cell infiltration in osteoarthritis (OA).

Methods:Two eligible datasets (GSE63359 and GSE48556) were obtained from the GEO database to discern differentially expressed genes (DEGs). The machine learning strategy was employed to filtrate diagnostic biomarkers for OA. Additional verification was implemented by collecting clinical samples of OA. The CIBERSORT website estimated relative subsets of RNA transcripts to evaluate the immune-inflammatory states of OA. The link between specific DEGs and clinical immune-inflammatory markers was found by correlation analysis.

Results:Overall, 67 robust DEGs were identified. The nuclear receptor subfamily 2 group C member 2 (NR2C2), transcription factor 4 (TCF4), stromal antigen 1 (STAG1), and interleukin 18 receptor accessory protein (IL18RAP) were identified as effective diagnostic markers of OA in peripheral blood. All four diagnostic markers showed significant increases in expression in OA. Analysis of immune cell infiltration revealed that macrophages are involved in the occurrence of OA. Candidate diagnostic markers were correlated with clinical immune-inflammatory indicators of OA patients.

Conclusion:We highlight that DEGs associated with immune inflammation (NR2C2, TCF4, STAG1, and IL18RAP) may be potential biomarkers for peripheral blood in OA, which are also associated with clinical immune-inflammatory indicators.

About the authors

Qiao Zhou

Department of Rheumatism Immunity,, The First Affiliated Hospital of Anhui University of Chinese Medicine,

Email: info@benthamscience.net

Jian Liu

Department of Rheumatism Immunity, Anhui University of Chinese Medicine

Author for correspondence.
Email: info@benthamscience.net

Ling Xin

Institute of Rheumatism Prevention and Treatment of Traditional Chinese Medicine, Anhui Academy of Chinese Medicine Sciences

Email: info@benthamscience.net

Yuedi Hu

Institute of Rheumatism Prevention and Treatment of Traditional Chinese Medicine,, Anhui Academy of Chinese Medicine Sciences

Email: info@benthamscience.net

Yajun Qi

Institute of Rheumatism Prevention and Treatment of Traditional Chinese Medicine, Anhui Academy of Chinese Medicine Sciences

Email: info@benthamscience.net

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