Assessment of the toxic effect of the medical glue «Sulfacrylate» in M-22 cell culture

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Abstract

Sulfacrylate is a medical glue used in general and vascular surgery, traumatology, maxillofacial surgery, thoracic and abdominal surgery, and dentistry. To connect tissues in plastic ophthalmic surgery, the toxic properties of Sulfacrylate should be studied.

AIM: To evaluate the toxic effect of Sulfacrylate in human cell culture in vitro.

MATERIAL AND METHODS: In vitro studies were conducted on human fibroblast cultures of the M-22 line from the 20th passage. Sulfacrylate was used in the present study (registration certificate for a medical device no. FSR 2010/09805; December 31, 2010). A lyophilized bandage based on human type 1 collagen was used to fix the glue. The total number of cells at the bottom of wells, cell density at the bottom of wells and on collagen dressings (1 thousand cells per cm2), structural integrity of cells and their morphology, integrity of cell membranes were evaluated.

RESULTS: The toxic effect of collagen dressings with Sulfacrylate was limited by the size of the bandage. Collagen dressings with Sulfacrylate did not affect the viability of cells not in direct contact with the dressings and did not cause a pronounced decrease in the proliferative activity of cells. The minimum optimal dosage of adhesive for plastic ophthalmic surgery was 1–3 µkl.

CONCLUSION: The use of Sulfacrylate in plastic ophthalmic surgery is feasible; however, the volume used should be minimized.

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About the authors

Irina A. Filatova

Helmholtz National Medical Research Center for Eye Diseases

Email: filatova13@yandex.ru
ORCID iD: 0000-0001-5449-4980
SPIN-code: 1797-9875

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Moscow

Olesya V. Soldatkina

Helmholtz National Medical Research Center for Eye Diseases

Author for correspondence.
Email: olesya.sold@mail.ru

MD, graduate student

Russian Federation, Moscow

Maxim S. Makarov

N.V. Sklifosovsky Research Institute for Emergency Medicine

Email: makarovms@sklif.mos.ru
SPIN-code: 3543-5800

MD, researcher

Russian Federation, Moscow

Natalya V. Borovkova

N.V. Sklifosovsky Research Institute for Emergency Medicine; Russian Medical Academy of Continuous Professional Education; Pirogov Russian National Research Medical University

Email: borovkovanv@sklif.mos.ru
ORCID iD: 0000-0002-8897-7523
SPIN-code: 9339-2800

MD, Dr. Sci. (Medicine)

Russian Federation, Moscow; Moscow; Moscow

Maya V. Storozheva

N.V. Sklifosovsky Research Institute for Emergency Medicine

Email: mayya.storozheva@yandex.ru
ORCID iD: 0000-0003-1927-2404
SPIN-code: 7789-3277

MD, researcher

Russian Federation, Moscow

Ivan N. Ponomarev

N.V. Sklifosovsky Research Institute for Emergency Medicine

Email: rzam@yandex.ru
ORCID iD: 0000-0002-2523-6939
SPIN-code: 4705-9314

MD, Cand. Sci. (Medicine)

Russian Federation, Moscow

References

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2. Fig. 1. Human fibroblasts of the M-22 line after 3 days of cultivation in the absence of (a, a') and in the presence of (b, b') Sulfacrylate glue (2 µkl on a 0.25 cm2 collagen bandage), vital staining with tripaflavin–rhodamine C: a, b — increase in x40; a', b' — increase in x200. Dotted line: border of collagen dressings with Sulfacrylate glue; arrow: the centers of active growth of M-22 cells.

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3. Fig. 2. Detection of human fibroblasts of the M-22 line on the surface of collagen dressings after 3 days of cultivation. Vital staining with tripaflavin–rhodamine C, increase x40: a — bandages without Sulfacrylate glue; b — bandages with Sulfacrylate glue (2 µl of glue on a 0.25 cm2 collagen bandage).

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