Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report

Achimi Akoji; Gideon Ayeni; Larayetan Rotimi; Yahaya Abdulrazaq; Emmanuel Titus; Sule Ajuma; Audu Jamila; Ajayi Abayomi; Egwumah Christian; Ogohi Agah; Ogundipe Emmanuel; Audu Faruk; Orogun Moradeke; Monday Sylvester; Ogejah Zuleihat; Wulapba Dauda

doi:10.2174/0122127968284847240920103341

Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report

Authors: Akoji A.¹, Ayeni G.¹, Rotimi L.², Abdulrazaq Y.², Titus E.³, Ajuma S.¹, Jamila A.¹, Abayomi A.⁴, Christian E.¹, Agah O.¹, Emmanuel O.⁵, Faruk A.¹, Moradeke O.¹, Sylvester M.¹, Zuleihat O.¹, Dauda W.¹
Affiliations:
1. Department of Biochemistry, Prince Abubakar Audu University
2. Department of Pure and Industrial Chemistry, Prince Abubakar Audu University
3. Department of Medical Biochemistry, Faculty of Basic Medical Science, College of Health Sciences, Prince Abubakar Audu University
4. Department of Anatomy, Faculty of Basic Medical Science, College of Health Sciences, Prince Abubakar Audu University
5. Department of Biochemistry and Molecular Biology, Federal University
Issue: Vol 18, No 2 (2024)
Pages: 71-83
Section: Biochemistry
URL: https://ruspoj.com/2212-7968/article/view/643881
DOI: https://doi.org/10.2174/0122127968284847240920103341
ID: 643881

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Abstract

Objective:Nanoparticles (NPs) are reliable biological tools for curative purposes through their application in nanomedicine. The present study synthesized and characterized silver nanoparticles (AgNPs) from Tetrapetra tetrapleura fruit. The investigation aims to examine the antidiabetic effect of the AgNPs using in vitro and in vivo models.

Methods:Briefly, the synthesized AgNPs were confirmed by the application of ultraviolet-visible (UV-Vis) spectroscopy, and five other techniques, viz; transmission electron microscopy (TEM) techniques, Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction analysis (XRD) and scanning electron microscope (SEM). The in vitro model assay investigated the scavenging effect of AgNPS on 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (O2ˉ), hydroxyl anion (-OH), ferric reducing antioxidant power (FRAP), and α-amylase/α-glucosidase inhibitory activity. The in vivo model involving rats-induced type-2 diabetes with streptozotocin (STZ) was divided into six (6) groups of seven (7) rats each to assess antioxidative parameters.

Results:The AgNPs scavenged free radicals (DPPH) and moderately inhibited (O2ˉ), hydroxyl anion (-OH), reduced ferric to ferrous ions, and inhibited both α-amylase and α-glucosidase activity with increasing concentrations. Similarly, AgNPs ameliorated oxidative stress imposed by type 2 diabetes on the rats tissues significantly (p < 0.05), depleting total cholesterol, low-density lipoprotein (LDL), and increased total protein composite and high-density lipoprotein (HDL) contents. The AgNPs enhanced catalase and superoxide dismutase, reduced glutathione (GSH), and, concomitantly, decreased malondialdehyde (MDA) levels in the tissue homogenate.

Conclusion:These findings provide scientific evidence for the first time, finding the application of a biogenic compound synthesized from T. tetrapleura fruit in the treatment of type 2 diabetes.

Keywords

Nanoparticles, <, i&, gt, T. tetrapleura&, lt, /i&, gt, spectroscopy, streptozotocin, type 2- diabetes, inhibition, scavenging.

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Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report

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