FUNCTIONAL DIVERSIFICATION OF THE CAROTENOID- CIS-TRANS -ISOMERASES CrtISO, CrtISO-L1, AND CrtISO-L2 IN TOMATO SPECIES ( SOLANUM , SECTION LYCOPERSICON)

G. I. Efremov; Ефремов Г. И.; A. V. Shchennikova; Щенникова А. В.; E. Z. Kochieva; Кочиева Е. З.

doi:10.31857/S2686738922600686

FUNCTIONAL DIVERSIFICATION OF THE CAROTENOID-CIS-TRANS-ISOMERASES CrtISO, CrtISO-L1, AND CrtISO-L2 IN TOMATO SPECIES (SOLANUM, SECTION LYCOPERSICON)

Authors: Efremov G.I.¹, Shchennikova A.V.¹, Kochieva E.Z.¹
Affiliations:
1. Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences"
Issue: Vol 508, No 1 (2023)
Pages: 9-13
Section: Articles
URL: https://ruspoj.com/2686-7389/article/view/651142
DOI: https://doi.org/10.31857/S2686738922600686
EDN: https://elibrary.ru/MOKOFP
ID: 651142

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Abstract

The expression of the genes of carotenoid-cis-trans isomerases CrtISO, CrtISO-L1 and CrtISO-L2 was studied in comparison with the content of carotenoids in tomato species with different ripe fruit colors: green (Solanum habrochaites), yellow (S. cheesmaniae) and red (S. pimpinellifolium and S. lycopersicum). More ancient origin of CrtISO-L2 was shown in relation to CrtISO and CrtISO-L1. A similar content of total carotenoids (leaves) and β-carotene (ripe fruits) was found between the samples. Unlike fruits of S. habrochaites and S. cheesmaniae, red fruits accumulated lycopene and 20-30 times more total carotenoids. The highest level of transcripts both in leaves and in ripe fruits was detected for CrtISO. The CrtISO-L1 and CrtISO-L2 were transcribed high in leaves and low in fruits, except for the high expression of CrtISO-L2 in S. lycopersicum fruits. No relationship was observed between the content of carotenoids and the level of gene expression in the fruit. In the leaves, a positive correlation between the amount of carotenoids and the levels of CrtISO-L1 and CrtISO-L2 transcripts was found.

Keywords

tomato species, Solanum, carotenogenesis, carotenoid-cis-trans-isomerase, evolution of tomato fruit color

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