Safety of agricultural products: multicomponent determination of pesticide residues in cereals

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Abstract

Introduction. Cereal grain is an important part of the human daily diet and belongs to a large number of food items produced and consumed throughout the world. Cereals are cultivated using a long list of chemical plant protection products (herbicides, insecticides, fungicides, growth regulators). In this connection, the analysis of pesticide residues, including optimization, development of monitoring methods, and programs, is of particular importance.

Purpose of the work. To create a multicomponent method for determining residual amounts of pesticides of various chemical classes and their metabolites in cereals.

Material and methods. The identification and quantitative determination of the active substances of pesticides of various classes (neonicotinoids, triazoles, imidazoles, pyrethroids, organophosphorus compounds, strobilurins, etc.) in the joint presence were performed by methods based on the use of liquid and gas-liquid chromatography with mass spectrometric detection. The QuEChERS technology was used as a sample preparation method, based on the extraction of pesticides with an organic solvent from a homogenized grain sample in the presence of salts containing citrate buffer and purification of the extracts from polar compounds by solid-state dispersion extraction.

Results. The developed multicomponent method for determining the residual amounts of pesticides of various chemical classes and their metabolites (40 items in total) in cereals was used to control the levels of xenobiotics studied in wheat, barley and corn samples produced in various regions of the Russian Federation (Moscow, Leningrad, Orel, Omsk, Voronezh, Ryazan, Rostov, Volgograd regions, Krasnodar Area), as well as in rice grain (the country of origin is the Socialist Republic of Vietnam) purchased using a consumer buying process. The identified levels of individual active substances were significantly lower than the established values of the maximum allowable level.

Conclusion. The developed method is aimed at methodological support of food safety control.

About the authors

Valerii N. Rakitskii

F.F. Erisman Federal Scientific Center of Hygiene, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Author for correspondence.
Email: noemail@neicon.ru
ORCID iD: 0000-0002-9959-6507
Russian Federation

Nataliia E. Fedorova

F.F. Erisman Federal Scientific Center of Hygiene, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: analyt1@yandex.ru
ORCID iD: 0000-0001-8278-6382

MD, Ph.D., DSci., Head of the department of analytical control methods, F. Erisman Federal Scientific Center of Hygiene, Federal Service for Supervision of Consumer Rights Protection and Human Welfare, 141014, Mytishchi, Moscow region, Russian Federation.

e-mail: analyt1@yandex.ru

Russian Federation

Olga E. Egorchenkova

F.F. Erisman Federal Scientific Center of Hygiene, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4653-6970
Russian Federation

Marina S. Grechina

F.F. Erisman Federal Scientific Center of Hygiene, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: noemail@neicon.ru
ORCID iD: 0000-0003-3324-5090
Russian Federation

Alena V. Suslova

F.F. Erisman Federal Scientific Center of Hygiene, Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: noemail@neicon.ru
ORCID iD: 0000-0001-8181-4409
Russian Federation

References

  1. Mel’nikov N.N. Pesticides. Chemistry, Technology and Application [Khimiya, tekhnologiya i primenenie]. Moscow: Khimiya; 1987. (in Russian)
  2. Pimentel D. Environmental aspects of pest management, chemistry and world supplies. In: Shemilt L.W., ed. Chemistry and World Food Supplies: The New Frontiers, Chemarawn II. Oxford: Pergamon Press; 1983: 185–201.
  3. Antonovich E.A., Sedokur L.K. The Quality of Food in the Context of the Chemicalization of Agriculture. Handbook [Kachestvo produktov pitaniya v usloviyakh khimizatsii sel’skogo khozyaystva. Spravochnik]. Kiev: Urozhay; 1990. (in Russian)
  4. Rodenburg J., Riches C.R., Kayeke J.M. Addressing current and future problems of parasitic weeds in rice. Crop Protection. 2009; 29(3): 210–21. https://doi.org/10.1016/j.cropro.2009.10.015
  5. Rakitskiy V.N. Toxicology of pesticides. Toksikologicheskiy vestnik. 2010; (3): 21–3. (in Russian)
  6. Tutel’yan V.A., Khotimchenko S.A. Quality and safety of food products: modern aspects. In: Materials of the All-Russian Scientific-Practical Conference with International Participation «Hygiene, Toxicology, Occupational Pathology: Traditions and Modernity» [Materialy Vserossiyskoy nauchno-prakticheskoy konferentsii s mezhdunarodnym uchastiem «Gigiena, toksikologiya, profpatologiya: traditsii i sovremennost’»]. Mytishchi; 2016: 242–8. (in Russian)
  7. Handbook of pesticides and agrochemicals approved for use in the Russian Federation. Moscow: Listerra; 2020. (in Russian)
  8. Lehotay S.J. QuEChERS sample preparation approach for mass spectrometric analysis of pesticide residues in foods. Methods Mol. Biol. 2011; 747: 65–91. https://doi.org/10.1007/978-1-61779-136-9_4
  9. Bruzzoniti M.C., Checchini L., De Carlo R.M., Orlandini S., Rivoira L., Del Bubba M. QuEChERS sample preparation for the determination of pesticides and other organic residues in environmental matrices: a critical review. Anal. Bioanal. Chem. 2014; 406(17): 4089–116. https://doi.org/10.1007/s00216-014-7798-4
  10. Mastovska K., Dorweiler K.J., Lehotay S.J., Wegfsheid J.S., Szpylka K.A. Pesticide multiresidue analysis in cereal grains using modified QuEChERS method combined with automated direct sample introduction GC-TOFMS and UPLC-MS/MS techniques. J. Agric. Food Chem. 2010; 58(10): 5959–72. https://doi.org/10.1021/jf9029892
  11. He Z., Wang L., Peng Y., Luo M., Wang W., Liu X. Multiresidue analysis of over 200 pesticides in cereals using a QuEChERS and gas chromatography-tandem mass spectrometry-based method. Food Chem. 2015; 169: 372–80. https://doi.org/10.1016/j.foodchem.2014.07.102
  12. Balinova A., Mladenova R., Shtereva D. Solid phase extraction on sorbents of different retention mechanisms followed by determination by gas chromatography mass spectrometric and gas chromatograpgy electron capture detection of pesticide residues in crops. J. Chromatogr. A. 2007; 1150(1–2): 136–44. https://doi.org/10.1016/j.chroma.2007.02.002
  13. Boccacci-Mariani M., D’Aiuto V., Giannetti V. Multiresidue method for the determination of organophosphorus pesticides in cereal matrixes. J. AOAC Int. 2010; 93(3): 999–1006. https://doi.org/10.1093/jaoac/93.3.999
  14. Anastassiades M., Lehotay S.J., Stajnbaher D., Schenck F.J. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce. J. AOAC Int. 2003; 86(2): 412–31. https://doi.org/10.1093/jaoac/86.2.412
  15. Gonzalez-Curbelo M.A., Herrera-Herrera A.V., Ravelo-Perez L.V., Hernandes-Borges J. Sample-preparation methods for pesticide-residue analysis in cereals and derivatives. Trends Anal. Chem. 2012; 38: 32–51. https://doi.org/10.1016/j.trac.2012.04.010.
  16. Mondal R., Kole R.K., Bhttacharyya A. Validation of multiresidue method for analysis of 31 pesticides in rice using gas chromatography-tandem mass spectrometry. J. AOAC Int. 2017; 100(4): 1094–101. https://doi.org/10.5740/jaoacint.16-0377
  17. Nguyen T.D., Han E.M., Seo M.S., Kim S.R., Yun M.Y., Lee D.M., et al. A multi-residue method for the determination of 203 pesticides in rice paddies using gas chromatography/mass spectrometry. Anal. Chim. Acta. 2008; 619(1): 67–74. https://doi.org/10.1016/j.aca.2008.03.031
  18. Leandro C.C., Hancock P., Fussell R.J., Keely B.J. Comparison of ultraperformance liquid chromatography and high-performance liquid chromatography for the determination of priority pesticides in baby foods by tandem quadrupole mass spectrometry. J. Chromatogr. A. 2006; 1103(1): 94–101. https://doi.org/10.1016/j.chroma.2005.10.077
  19. Nguen T.D., Lee B.S., Lee B.R., Lee D.M., Lee G.H. A multiresidue method for the determination of 109 pesticides in rice using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) sample preparation method and gas chromatography/mass spectrometry with temperature control and vacuum concentration. Rapid Commun. Mass Spectrom. 2007; 21(18): 3115–22. https://doi.org/10.1002/rcm.3199
  20. Paya’ P., Anastassiades M., Mack D., Sigalova I., Tasdelen B., Oliva J., et al. Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) pesticide multiresidue method in combination with gas and liquid chromatography and tandem mass spectrometric detection. Anal. Bioanal. Chem. 2007; 389(6): 1697–714. https://doi.org/10.1007/s00216-007-1610-7
  21. Mol H.G., Plaza-Bolaños P., Zomer P., de Rijk T.C., Stolker A.A., Mulder P.P. Toward a generic extraction method for simultaneous determination of pesticides, mycotoxins, plant toxins, and veterinary drugs in feed and food matrixes. Anal. Chem. 2008; 80(24): 9450–9. https://doi.org/10.1021/ac801557f
  22. ЕС № SANTE/12682/2019. Guidance document on analytical quality control and method validation procedures for pesticides residues analysis in food and feed. Imp. 2020-01-01; 2020.
  23. SANCO/825/00. European Commission: Guidance Document on Pesticide Residue Analytical Methods. Imp. 2010-11-16; 2010.
  24. Andoralov A.M., Kuznetsov S.V., Afonin M.B. Matrix effect in the analysis of residual amounts of pesticides in fruits and vegetables by high performance liquid chromatography with quadrupole time-of-flight mass spectrometric detection. Nauchno-tekhnicheskiy vestnik Bryanskogo gosudarstvennogo universiteta. 2017; (1): 9–17. (in Russian)
  25. Takatori S., Okihashi M., Okamoto Y., Kitagawa Y., Kakimoto S., Murata H., et al. A rapid and easy multiresidue method for the determination of pesticide residues in vegetables, fruits, and cereals using liquid Chromatography/tandem mass spectrometry. J. AOAC Int. 2008; 91(4): 871–83. https://doi.org/10.5772/14008
  26. Koesukwiwat U., Lehotay S.J., Mastovska K., Dorweller K.J., Leepipatpiboon N. Extension of the QuEChERS method for pesticide residues in cereals to flaxseeds, peanuts, and doughs. J. Agric. Food Chem. 2010; 58(10): 5950–8. https://doi.org/10.1021/jf902988b
  27. Food and Agriculture Organization of the United Nations. Available at: http://faostat.fao.org

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Copyright (c) 2024 Rakitskii V.N., Fedorova N.E., Egorchenkova O.E., Grechina M.S., Suslova A.V.


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