Creation of Induced Pluripotent Stem Cells ICGi044-B and ICGi044-C Using Reprogramming of Peripheral Blood Mononuclear Cells of a Patient with Parkinson’s Disease Associated with  с.1492T&gt;G  Mutation in the  GLUD2  Gene

D. A. Sorogina; Сорогина Д. А.; E. V. Grigor’eva; Григорьева Е. В.; A. A. Malakhova; Малахова А. А.; S. V. Pavlova; Павлова С. В.; S. P. Medvedev; Медведев С. П.; Y. V. Vyatkin; Вяткин Ю. В.; E. A. Khabarova; Хабарова Е. А.; J. A. Rzaev; Рзаев Дж. А.; S. M. Zakian; Закиян С. М.

doi:10.31857/S0475145023010123

Creation of Induced Pluripotent Stem Cells ICGi044-B and ICGi044-C Using Reprogramming of Peripheral Blood Mononuclear Cells of a Patient with Parkinson’s Disease Associated with с.1492T>G Mutation in the GLUD2 Gene

Authors: Sorogina D.A.¹^,2, Grigor’eva E.V.¹, Malakhova A.A.¹, Pavlova S.V.¹, Medvedev S.P.¹, Vyatkin Y.V.², Khabarova E.A.¹^,3, Rzaev J.A.³, Zakian S.M.¹
Affiliations:
1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
2. Novosibirsk State University
3. FSBI Federal Neurosurgical Center
Issue: Vol 54, No 1 (2023)
Pages: 114-122
Section: Collection of pluripotent stem cell lines
URL: https://ruspoj.com/0475-1450/article/view/669976
DOI: https://doi.org/10.31857/S0475145023010123
EDN: https://elibrary.ru/FRVDZS
ID: 669976

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Abstract

Parkinson’s disease is a multifactorial disease; both genetic predisposition (5% of all cases), environmental factors and age-related changes in the brain and other body systems contribute to its etiology. For the diagnosis and study of the pathology of the development of the disease, it is important to search for new polymorphisms associated with hereditary forms of the disease. We analyzed the clinical exome of a 55-year-old patient with Parkinson’s disease and identified a single nucleotide polymorphism in the GLUD2 gene (c.1492T>G). This genetic variant is pathogenic according to the ClinVar database, but the mechanism of pathogenesis is still poorly understood. In addition, there are currently no relevant models based on human cells, which is of great interest. We generated induced pluripotent stem cells (iPSCs) from patient peripheral blood mononuclear cells using non-integrating episomal vectors expressing OCT4, KLF4, L‑MYC, SOX2, LIN28, and p53 shRNA. The obtained iPSC lines (ICGi044-B and ICGi044-C) demonstrate typical ESC-like morphology, normal karyotype (46,XY), express pluripotency markers (OCT4, SOX2, NANOG, SSEA4, TRA-1-60) and are able to give derivatives of three germ layers. The iPSC lines ICGi044-B and ICGi044-C, as well as their neural derivatives, represent an unique in vitro cell model for studying the pathogenetic mechanisms of the development of Parkinson’s disease associated with the c.1492T>G mutation in the GLUD2 gene.

Keywords

reprogramming, induced pluripotent stem cells, Parkinson’s disease, polymorphisms

References

Choppa P.C., Vojdani A., Tagle C. et al. Multiplex PCR for the detection of Mycoplasma fermentans, M. hominis and M. penetrans in cell cultures and blood samples of patients with chronic fatigue syndrome // Mol. Cell Probes. 1998. V. 12(5). P. 301–308. https://doi.org/10.1006/mcpr.1998.0186
Cowan C.A., Klimanskaya I., McMahon J. et al. Derivation of embryonic stem-cell lines from human blastocysts // N. Engl. J. Med. 2004. V. 350(13). P. 1353–1356. https://doi.org/10.1056/NEJMsr040330
Grigor’eva E.V., Malankhanova T.B., Surumbayeva A. et al. Generation of GABAergic striatal neurons by a novel iPSC differentiation protocol enabling scalability and cryopreservation of progenitor cells // Cytotechnology. 2020. V. 72(5). P. 649–663. https://doi.org/10.1007/s10616-020-00406-7
Grigor’eva E.V., Kopytova A.E., Yarkova E.S., Pavlova S.V., Sorogina D.A., Malakhova A.A., Malankhanova T.B., Baydakova G.V., Zakharova E.Y., Medvedev S.P., Pchelina S.N., Zakian S.M. Biochemical characteristics of iPSC-derived dopaminergic neurons from N370S GBA variant carriers with and without Parkinson’s disease // Int. J. Mol. Sci. 2023. V. 24(5). P. 4437. https://doi.org/10.3390/ijms24054437.
Okita K., Yamakawa T., Matsumura Y. et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood // Stem Cells. 2013. V. 31. № 3. P. 458–466.